Diagnosis of Crystalopathies via Raman Spectroscopy

通过拉曼光谱诊断晶体病

基本信息

  • 批准号:
    7276958
  • 负责人:
  • 金额:
    $ 6.93万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-08-25 至 2008-07-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Misdiagnosis of the presence and the identity of crystal species in joint space are often penalized with the incorrect treatment of the arthropathic condition. The existing diagnostic standard of polarized imaging lacks specificity and sensitivity and it is largely limited to microscopically visible birefringent crystals. In the current study we propose Raman spectroscopy as an alternative for diagnostic technology for improved identification of crystal species in joint spaces. In Raman spectroscopy molecular vibrations are triggered through laser excitation and the reflected light holds information on the identity as well as on the amount of species in the observed region. The technique is currently utilized for arthroscopic in vivo diagnosis of other diseases; thus, based on this clinical potential it would be worthwhile to explore its analytical and clinical feasibility towards the assessment of crystal species involved in arthropathic conditions. The first phase of the proposed exploratory study will reveal the identification thresholds of various crystals that can be detected by Raman spectroscopy. Synthetic analogs of monosodium urate, calcium pyrophosphate dihydrate, hydroxyapatite, octacalcium phosphate, tricalcium phosphate, calcium oxalate, cholesterol, cystine and xanthine will be mixed with healthy synovial fluid samples at various concentrations and these mixtures will be analyzed at the macro level to detect the cumulative signal emanating from crystals. We will attempt to lower the identification thresholds by employing surf ace-enhanced Raman spectroscopy and by principal component analysis of the spectral data. During the second phase synovial fluid samples will be taken from patients and the presence or absence of crystals will be confirmed via compensated polarized microscopy, X-ray diffraction and alizarin staining. Aliquots of these samples will be subjected to Raman analysis to determine whether Raman analysis can detect the presence and identity of crystals in clinical samples. If this exploratory study demonstrates that the analytical limits of Raman analysis suffice to cover clinical concentrations of crystal species than comprehensive clinical studies can be conducted to assess the specificity and the sensitivity of the technique finally leading to the development of an arthroscopic Raman device.
描述(由申请人提供):关节间隙中晶体物质的存在和身份的误诊通常会导致关节病状况的不正确治疗。现有的偏振成像诊断标准缺乏特异性和敏感性,并且在很大程度上限于显微镜下可见的双折射晶体。在目前的研究中,我们提出了拉曼光谱作为一种替代诊断技术,以提高识别的晶体物种在联合空间。在拉曼光谱中,分子振动通过激光激发而触发,并且反射光保持关于观察区域中的种类的身份以及数量的信息。该技术目前用于关节镜下体内诊断其他疾病;因此,基于这种临床潜力,值得探索其分析和临床可行性,以评估关节病条件中涉及的晶体种类。拟议的探索性研究的第一阶段将揭示可以通过拉曼光谱检测的各种晶体的识别阈值。将不同浓度的尿酸盐、焦磷酸钙二水合物、羟基磷灰石、磷酸八钙、磷酸三钙、草酸钙、胆固醇、胱氨酸和黄嘌呤的合成类似物与健康滑液样品混合,并在宏观水平上分析这些混合物,以检测晶体发出的累积信号。我们将尝试通过采用表面增强拉曼光谱和光谱数据的主成分分析来降低识别阈值。在第二阶段,将从患者身上采集滑液样本,并通过补偿偏振显微镜、X射线衍射和茜素染色来确认晶体的存在或不存在。将对这些样品的等分试样进行拉曼分析,以确定拉曼分析是否可以检测临床样品中晶体的存在和鉴别。如果该探索性研究证明拉曼分析的分析限度足以涵盖晶体物质的临床浓度,则可以进行全面的临床研究以评估该技术的特异性和灵敏度,最终导致关节镜拉曼器械的开发。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Ozan Akkus其他文献

Ozan Akkus的其他文献

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{{ truncateString('Ozan Akkus', 18)}}的其他基金

Cartilage Repair by Condensed Mesenchymal Stem Cell Delivery via Collagen Fabric
通过胶原蛋白织物输送浓缩间充质干细胞来修复软骨
  • 批准号:
    9441710
  • 财政年份:
    2017
  • 资助金额:
    $ 6.93万
  • 项目类别:
Tendon Tissue Engineering by Electrochemically Aligned Collagen Bioscaffolds
通过电化学排列胶原生物支架进行肌腱组织工程
  • 批准号:
    9089701
  • 财政年份:
    2015
  • 资助金额:
    $ 6.93万
  • 项目类别:
Tendon Tissue Engineering by Electrochemically Aligned Collagen Bioscaffolds
通过电化学排列胶原生物支架进行肌腱组织工程
  • 批准号:
    8835033
  • 财政年份:
    2014
  • 资助金额:
    $ 6.93万
  • 项目类别:
Tendon Tissue Engineering by Electrochemically Aligned Collagen Bioscaffolds
通过电化学排列胶原生物支架进行肌腱组织工程
  • 批准号:
    8697319
  • 财政年份:
    2014
  • 资助金额:
    $ 6.93万
  • 项目类别:
Tendon Tissue Engineering by Electrochemically Aligned Collagen Bioscaffolds
通过电化学排列胶原生物支架进行肌腱组织工程
  • 批准号:
    9247755
  • 财政年份:
    2014
  • 资助金额:
    $ 6.93万
  • 项目类别:
Diagnosis of Crystal-Based Arthropathies via Raman Spectroscopy
通过拉曼光谱诊断晶体关节病
  • 批准号:
    8322612
  • 财政年份:
    2011
  • 资助金额:
    $ 6.93万
  • 项目类别:
Diagnosis of Crystal-Based Arthropathies via Raman Spectroscopy
通过拉曼光谱诊断晶体关节病
  • 批准号:
    8187630
  • 财政年份:
    2011
  • 资助金额:
    $ 6.93万
  • 项目类别:
Diagnosis of Crystal-Based Arthropathies via Raman Spectroscopy
通过拉曼光谱诊断晶体关节病
  • 批准号:
    8528336
  • 财政年份:
    2011
  • 资助金额:
    $ 6.93万
  • 项目类别:
Electrochemically Guided Collagen Synthesis for Functional Tissue Engineering
用于功能组织工程的电化学引导胶原蛋白合成
  • 批准号:
    7691366
  • 财政年份:
    2008
  • 资助金额:
    $ 6.93万
  • 项目类别:
Electrochemically Guided Collagen Synthesis for Functional Tissue Engineering
用于功能组织工程的电化学引导胶原蛋白合成
  • 批准号:
    7587640
  • 财政年份:
    2008
  • 资助金额:
    $ 6.93万
  • 项目类别:

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