Antigen Detection Assay for Tuberculosis

结核病抗原检测分析

• 批准号: 7157546
• 负责人: Antonio Campos-Neto
• 金额: $ 38.7万
• 依托单位: INBIOS INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7157546
• 关键词: antigens; clinical research; diagnosis; proteins; recombinant proteins; tuberculosis; urine

DESCRIPTION (provided by applicant): Antigen detection assay in contrast to conventional serological test, detects disease status and not the host antibody response to the disease etiological agent. It can therefore, be used for both diagnosis and disease treatment follow-up purposes. Antigen detection assay has recently been successfully introduced for the diagnosis of different infectious diseases including hepatitis, pneumonia caused by Streptococcus pneumoniae or Legionella pneumophilla, sore throat cause by Streptococcus pyogenes, and amoebiasis. Although antigen detection assay has the potential to discriminate latent from adult tuberculosis, paradoxically this test has not yet been developed for this disease. Using the ultra-sensitive mass spectrometry technology we have recently described the presence of M. tuberculosis proteins in urine of infected mice. The proteins were sequenced, the encoding genes were cloned, and the recombinant proteins were obtained. 1 such molecule revealed to be a promising tool for the diagnosis of tuberculosis. Using similar approach we have recently identified M. tuberculosis antigens in urine of human patients with pulmonary tuberculosis. The present project aims to produce the corresponding recombinant proteins and use them as well as the ones previously described in mouse model to develop antigen detection assays for the diagnosis of tuberculosis disease.

描述(申请人提供)：抗原检测试验与传统的血清学试验不同，检测的是疾病状态，而不是宿主抗体对疾病病因的反应。因此，它既可用于诊断，也可用于疾病治疗随访。最近，抗原检测方法被成功地引入到不同传染病的诊断中，包括肝炎、由肺炎链球菌或嗜肺军团菌引起的肺炎、由化脓性链球菌引起的咽痛和阿米巴病。虽然抗原检测试验有可能区分潜伏性结核病和成人结核病，但矛盾的是，这种检测方法还没有被开发出来用于这种疾病。利用超灵敏的质谱学技术，我们最近描述了在感染的小鼠的尿液中存在结核分枝杆菌蛋白。对重组蛋白进行测序，克隆编码基因，获得重组蛋白。1这种分子被认为是诊断结核病的一种很有前途的工具。使用类似的方法，我们最近在人类肺结核患者的尿液中鉴定出结核分枝杆菌抗原。本项目的目的是生产相应的重组蛋白，并使用它们以及先前在小鼠模型中描述的蛋白来开发用于诊断结核病的抗原检测方法。