Tools for Genomic Investigations of Plasmodium vivax

间日疟原虫基因组研究工具

• 批准号: 7145646
• 负责人: AKHIL B VAIDYA
• 金额: $ 26.25万
• 依托单位: DREXEL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7145646
• 关键词: Plasmodium; genome; human; malaria; oligonucleotides

DESCRIPTION (provided by applicant): Of the four species causing human malaria, Plasmodium falciparum and P. vivax are responsible for more than 90% of the cases. Although much less prevalent than earlier times, vivax malaria is estimated to affect 70-80 million people each year, mainly in Asia, the Americas, and the Western Pacific region, thus making it the most widespread Plasmodium species. Investigations on P. vivax have lagged significantly because of the unavailability of facile tools for studying this parasite. The genome sequence of P. vivax is about to be completed at 10x coverage. However, tools to take advantage of the genomic sequence for molecular genetic studies are not available at present. To initiate such studies, this project will develop DNA microarray technology based on printed long oligonucleotide platform. Methods for optimal use of these microarrays will be developed and disseminated. These tools will be highly valuable in vivax malaria research as the field enters post-genomic era. Public Health Relevance: Malaria due to Plasmodium vivax is a major public health problem. Genomic sequencing of this parasite is now nearing completion, but tools to use this information to devise improved means to control vivax malaria are not available at present. This project aims to create such tools which will be made widely available to the research community.

描述（由申请人提供）：在引起人类疟疾的四种物种中，恶性疟原虫和杜瓦克斯疟原虫负责超过90％的病例。尽管据估计，据估计，据估计，Vivax疟疾的流行程度要少得多，但每年主要在亚洲，美洲和西太平洋地区影响70-80万人，因此使其成为最广泛的疟原虫物种。由于不容易研究该寄生虫的便利工具，对Vivax的调查显着滞后。维瓦克斯假单胞菌的基因组序列将在10倍覆盖范围内完成。但是，目前尚不提供利用用于分子遗传研究的基因组序列的工具。为了启动此类研究，该项目将基于印刷长寡核苷酸平台开发DNA微阵列技术。将开发和传播这些微阵列的最佳使用方法。随着该领域进入基因组时代，这些工具在体内疟疾研究中将是非常有价值的。公共卫生相关性：疟疾疟原虫疟原虫是一个主要的公共卫生问题。现在，该寄生虫的基因组测序已接近完成，但是目前尚不可用来使用此信息来设计这种信息来控制维瓦克斯疟疾的方法。该项目旨在创建此类工具，这些工具将为研究社区广泛使用。