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Tools for Genomic Investigations of Plasmodium vivax

间日疟原虫基因组研究工具

基本信息

批准号：
7232437
负责人：
AKHIL B VAIDYA
金额：
$ 14.57万
依托单位：
DREXEL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-06-01 至 2009-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7232437
关键词：
Address Affect Alternative Splicing Americas Antigens Applied Research Asia Biology Cessation of life Childhood Chloroquine resistance Communities Country DNA Microarray Chip DNA Microarray format Data Development Event Evolution Falciparum Malaria Fungal Genome Gene Expression Genes Genetic Variation Genome Genomics Glass Homo Human Institutes Investigation Knowledge Malaria Medicine Methodology Methods Microarray Analysis Modeling Molecular Genetics Molecular Profiling Mus Mutation Oligonucleotide Microarrays Oligonucleotides Parasites Patients Pharmaceutical Preparations Plasmodium Plasmodium vivax Primaquine Printing Protocols documentation Public Health RNA amplification Reagent Relapse Reporting Research Research Personnel Slide Spottings Standards of Weights and Measures Time Universities Variant Vivax Malaria Work base college design genome sequencing improved insight tool

项目摘要

DESCRIPTION (provided by applicant): Of the four species causing human malaria, Plasmodium falciparum and P. vivax are responsible for more than 90% of the cases. Although much less prevalent than earlier times, vivax malaria is estimated to affect 70-80 million people each year, mainly in Asia, the Americas, and the Western Pacific region, thus making it the most widespread Plasmodium species. Investigations on P. vivax have lagged significantly because of the unavailability of facile tools for studying this parasite. The genome sequence of P. vivax is about to be completed at 10x coverage. However, tools to take advantage of the genomic sequence for molecular genetic studies are not available at present. To initiate such studies, this project will develop DNA microarray technology based on printed long oligonucleotide platform. Methods for optimal use of these microarrays will be developed and disseminated. These tools will be highly valuable in vivax malaria research as the field enters post-genomic era. Public Health Relevance: Malaria due to Plasmodium vivax is a major public health problem. Genomic sequencing of this parasite is now nearing completion, but tools to use this information to devise improved means to control vivax malaria are not available at present. This project aims to create such tools which will be made widely available to the research community.

描述（由申请人提供）：在引起人类疟疾的四种物种中，恶性疟原虫和间日疟原虫占90%以上的病例。尽管间日疟的流行程度比以前低得多，但据估计，间日疟每年影响7000万至8000万人，主要在亚洲、美洲和西太平洋地区，因此使其成为最广泛的疟原虫物种。对间日疟原虫的研究已经明显滞后，因为没有研究这种寄生虫的简便工具。间日疟原虫的基因组测序即将在10倍覆盖率下完成。然而，利用基因组序列进行分子遗传学研究的工具目前还不可用。为了启动这些研究，本项目将开发基于打印长寡核苷酸平台的DNA微阵列技术。将开发和传播最佳使用这些微阵列的方法。随着间日疟研究进入后基因组时代，这些工具将对间日疟研究具有重要价值。公共卫生相关性：由间日疟原虫引起的疟疾是一个主要的公共卫生问题。这种寄生虫的基因组测序现已接近完成，但目前还没有利用这些信息设计改进的控制间日疟方法的工具。该项目旨在创建此类工具，供研究界广泛使用。