Characterising the genetic control of quality attributes in Pak Choi Brassica rapa subsp. Chinensis (L.).

1.组装资源的分子定义CN种子有大量的白菜种质用于其育种计划。对于这个新项目，我们将在JIC和Wageningen大学举办的汇编白菜/白菜核心种质和公开可获得的双亲种群来补充这些资源。这项iCASE奖项的第一阶段将采用高通量测序基因型别，以产生与最新的白菜型油菜基因组组合相一致的高密度基因数据。这些数据将用于探索种群亚结构和个体之间的关系，建立新的基于系统发育的遗传资源描述。将在新的和现有的种质中定义单倍型，提供一种跟踪导入片段的方法，加快先进回交系的创建，并提供一种在公司投资组合中定义知识产权的方法。开发改良育种途径组装的种质将以对品种发育和感官质量至关重要的形态性状为特征，例如农艺性状：植株形状、抽苔习性(开花时间)、茎裂开(基部)、柔软度；叶片质量：颜色、枯萎；收获后叶片变黄。这些性状的平均表型数据将与新的GBS SNP数据相结合，剖析这些性状的遗传规律，定义可利用的等位基因变异。这些数据将使种质资源的高效选择成为可能，并在CN Seed建立一个新的分子育种平台。

1. A molecular definition of assembled resourcesCN Seeds have a large collection of Pak Choi germplasm used in their breeding programme. For this new project, we will supplement these resources with assembled B. rapa/ Pak Choi core collections and publicly available bi-parental populations held at JIC and Wageningen University. The first phase of this ICASE award will employ high-throughput Genotype-By-Sequencing to generate high-density genotype data aligned with the latest B. rapa genome assembly. These data will be used to explore population sub-structure and relationships between individuals, to establish a new phylogeny-based description of the genetic resources. Haplotypes will be defined across new and existing germplasm, providing a means to track introgressed segments, speed up creation of advanced backcross lines, and provide a means to define intellectual property within the company's portfolio.2. Developing a pipeline for enhanced breedingThe assembled germplasm will be characterised for morphological traits key to varietal development and organoleptic quality, e.g., agronomic characters: plant shape, bolting habit (flowering time), stem cracking (base), suppleness; leaf quality: colour, wilting; post-harvest leaf yellowing. Mean phenotype data for these traits will be combined with new GBS SNP data to dissect the genetic regulation of these traits, define available allelic variation. These data will enable the efficient selection of germplasm and establish a new molecular breeding platform at CN Seeds.