MOUSE GENETICS CORE

小鼠遗传学核心

• 批准号: 6953880
• 负责人: BRUCE L TEMPEL
• 金额: $ 18.27万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6953880
• 关键词: biomedical facility; gene targeting; genetically modified animals; genetics; genotype; hearing disorders; laboratory mouse; mutant

Hearing loss is the most frequent sensory defect in humans. Congenital, perinatal or early onset hearing loss occurs in approximately 7 out of 1000 neonates in the United States. In 50% of these cases, a genetic contribution is known or suspected, the powerful molecular and genetic techniques available in mouse and zebra fish are useful model systems for studying the biology of hearing. The functional similarities between mouse and human audition make mouse a particularly attractive model for hearing loss observed clinically. In support of Users of mice to study auditory system function, Core D will provide expert mouse husbandry (receiving, setting up crosses, weaning, fostering, background transfers, etc), genotyping, and notification/delivery of requested mice to Users. Per diem charges for mouse care will be the responsibility of the User, provided that they have a funded project that includes support for mouse cage costs. To encourage innovative and new collaborative projects, we will provide per diem support for common control animals and for approved projects in funding transition. All services provided by Core D will be reviewed periodically to ensure quality and accessibility. The Genetics Core will provide services for a variety of Users. Efficiency of mouse care will be increased greatly by having centralized, expert mouse husbandry and genotyping. Users not proficient in mouse care will gain access the vast number of identified gene knockout mice and defined spontaneous mutants to further their research. Experienced mouse users will benefit from the familiarity of the Core staff with mouse behavior and care, providing better health and higher fecundity for their strains. Training in husbandry, genotyping, and behavioral characterization will be available to all Users and their lab personnel. To encourage interactions and new collaborations, information about the strains, techniques, and projects being pursued by all Users of the Core will be disseminated online and in Genetics Core User meetings.

听力损失是人类最常见的感觉缺陷。在美国的1000名新生儿中，大约7个发生了先天，围产期或早发性听力损失。在其中50％的情况下，遗传贡献是已知或怀疑的，在小鼠和斑马鱼中可用的强大分子和遗传技术是研究听力生物学的有用模型系统。小鼠和人类试镜之间的功能相似性使小鼠成为临床观察到的听力丧失的特别有吸引力的模型。为了支持小鼠的用户研究听觉系统功能，核心D将提供鼠标饲养专家（接收，设置十字架，断奶，促进，背景转移等），基因分型以及向用户提供所需的小鼠的通知/传递。每个Diem的鼠标护理费用将是用户的责任，前提是他们有一个资助的项目，其中包括对鼠标笼成本的支持。为了鼓励创新和新的合作项目，我们将为共同控制动物以及批准的资金过渡项目提供支持。核心D提供的所有服务将进行定期审查，以确保质量和可访问性。 遗传学核心将为各种用户提供服务。通过集中的，专家的小鼠饲养和基因分型，将大大提高小鼠护理的效率。不精通小鼠护理的用户将获得访问大量已识别的基因敲除小鼠，并定义了自发突变体以进一步进行研究。经验丰富的老鼠用户将受益于核心人员对鼠标行为和护理的熟悉，从而为他们的菌株提供更好的健康和更高的繁殖力。所有用户及其实验室人员将接受饲养，基因分型和行为表征的培训。鼓励互动和新的合作，有关应变，技术和项目的信息 所有核心用户都将在线和遗传学核心用户会议中传播。