CORE PROJECT

描述（由申请人提供）：这是约翰霍普金斯大学医学院听力与平衡中心向NIDCD申请研究核心资助的申请。该中心包括来自耳鼻咽喉-头颈外科和生物医学工程部门的11名研究人员。这些实验室提供了九个R 01奖项的研究基地，其科学目标从毛细胞的分子生物物理学到听力和平衡的行为测定。提出了三个研究核心，将促进正在进行的研究，并在现有方案之间的接口刺激新的举措。 分子生物学核心将提供分子遗传学研究工具的培训和使用机会，包括定量聚合酶链反应以及原位杂交和免疫组织学分子探针的生成和评估。一个“实时”荧光监测热循环仪将通过这个核心。 组织学中心将为光学和电子显微镜研究提供技术援助和材料支持。用户将获得组织准备、切片和可视化技术方面的指导。荧光显微镜和数字成像站将通过核心提供。 工程核心将开发和分发先进的数据采集和信号分析软件，并为中心实验室提供通用硬件和软件支持。 一个行政股将监督整个核心的业务，设计和维护一个中央网站，提供文书和人事支助，并编写报告。

DESCRIPTION (provided by applicant): This is an application from the Center for Hearing and Balance at Johns Hopkins University School of Medicine for a Research Core Grant from the NIDCD. The Center includes eleven investigators drawn from the departments of Otolaryngology- Head and Neck Surgery, and Biomedical Engineering. These laboratories provide a Research Base of nine R01 awards with scientific aims ranging from the molecular biophysics of hair cells to behavioral assays of hearing and balance. Three Research Cores are proposed that will facilitate ongoing research and stimulate new initiatives at the interface between existing programs. The Molecular Biology Core will provide training for and access to molecular genetic research tools including quantitative polymerase chain reaction and generation and assessment of molecular probes for in situ hybridization and immunohistology. A 'real-time' fluorescent monitor thermal cycler will be available through this Core. The Histology Core will provide technical assistance and material support for light and electron microscopic studies. Users will receive guidance in tissue preparation, sectioning, and visualization techniques. Fluorescence microscopy and a digital imaging station will be available through the Core. The Engineering Core will develop and distribute advanced data acquisition and signal analysis software, and provide general hardware and software support for Center laboratories. An Administrative unit will oversee operations throughout the Cores, design and maintain a central Website, provide clerical and personnel support and prepare reports.