Core Project

描述（由申请人提供）：这是约翰霍普金斯大学医学院听力和平衡中心的竞争性续期申请，用于国家耳聋和其他沟通障碍研究所的研究核心资助。该中心包括来自耳鼻咽喉头颈外科、生物医学工程、神经科学、神经病学、分子生物学和遗传学等部门的16名研究人员。这些实验室提供了20个R01奖项的研究基地，其中14个来自NIDCD，其科学目标从毛细胞的分子生物物理学到听力和平衡的行为测定。提出了三个研究核心，将促进正在进行的研究，并在现有方案之间的接口刺激新的举措。 1.工程核心将开发和分发先进的数据采集和信号分析软件，并为中心实验室提供通用硬件和软件支持。 2.组织学核心将为光学和电子显微镜研究提供技术援助和材料支持，包括为用户提供切片机和冷冻切片机。超薄切片和EM分析将由兼职高级组织学家提供。此外，用户将收到组织准备、切片和可视化技术方面的指导。荧光显微镜将可通过荧光显微镜和数字成像站在核心。 3.神经生理学核心将开发和分发先进的CMS记录方法，用于自由行为的动物。将在核心工作人员的协助下实施和应用留置多电极阵列。 4.此外，一个行政单位将监督整个核心的运作，组织中心的发言人计划，提供文书和人事支持，并准备报告。 中心管理 主任：约翰·列侬Fuchs

DESCRIPTION (provided by applicant): This is a competing renewal application from the Center for Hearing and Balance at Johns Hopkins University School of Medicine for a Research Core Grant from the National Institute for Deafness and Other Communication Disorders. The Center includes 16 investigators drawn from the Departments of Otolaryngology-Head and Neck Surgery, Biomedical Engineering, Neuroscience, Neurology, and Molecular Biology and Genetics. These laboratories provide a Research Base of 20 R01 awards, 14 of them from the NIDCD, with scientific aims ranging from the molecular biophysics of hair cells to behavioral assays of hearing and balance. Three Research Cores are proposed that will facilitate ongoing research and stimulate new initiatives at the interface between existing programs. 1. The Engineering Core will develop and distribute advanced data acquisition and signal analysis software, and provide general hardware and software support for Center laboratories. 2. The Histology Core will provide technical assistance and material support for light and electron microscopic studies, including access to microtomes and cryotomes for Users. Ultra-thin sectioning and EM analysis will be provided by a half-time senior histologist. Also, Users will receive guidance in tissue preparation, sectioning, and visualization techniques. Fluorescence microscopy will be available through a fluorescence microscope and digital imaging station in the Core. 3. The Neurophysiology Core will develop and distribute advanced CMS recording methods for use in freely-behaving animals. Indwelling multi-electrode arrays will be implemented and applied with the assistance of Core staff. 4. In addition, an Administrative unit will oversee operations throughout the Cores, organize the Center's speakers program, provide clerical and personnel support, and prepare reports. Center Administration Director: Paul A. Fuchs