Plasmodium carbohydrate receptors in Anopheles gambiae

冈比亚按蚊的疟原虫碳水化合物受体

• 批准号: 7154513
• 负责人: Rhoel David Ramos Dinglasan
• 金额: $ 4.88万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7154513
• 关键词: Anopheles; Plasmodium; carbohydrate receptor; enzymes; gene induction /repression; genes; genetics; glycoproteins; glycosylation; glycosyltransferase; infection; role; transferase

DESCRIPTION (provided by applicant): Carbohydrates and carbohydrate-binding proteins not only inhibit parasite attachment to the midgut brush border but completely block parasite development as well. Since the identity of the full range of polypeptide backbones that can bear these glycans is unknown, specific protein targeting is unfeasible. However, it is known that addition of specific glycans to polypeptides, such as mucins and other gut glycoproteins are regulated by a defined family of resident transferases in the gut Golgi. We hypothesize that ookinete attachment can be reduced or completely inhibited by interrupting glycosylation of midgut lumenal glycoproteins. To test this hypothesis, we envision a two-step process: 1) molecular identification and biochemical characterization of mosquito midgut enzymes involved in the glycosylation of midgut brush border glycoproteins, and 2) the use of reverse genetics (RNAi gene silencing) to assess the functional role of selected midgut-specific glycosylation enzymes on Plasmodium infection. This line of experimentation will open up new avenues of research through the basic, glycobiological analysis of the mosquito midgut biology.

描述（由申请人提供）：碳水化合物和碳水化合物结合蛋白不仅抑制寄生虫附着在中肠刷状缘上，而且还完全阻止寄生虫发育。由于可以承载这些聚糖的全部多肽主链的身份未知，因此特定的蛋白质靶向是不可行的。然而，众所周知，向多肽（例如粘蛋白和其他肠道糖蛋白）添加特定聚糖是由肠道高尔基体中特定的常驻转移酶家族调节的。我们假设可以通过中断中肠腔糖蛋白的糖基化来减少或完全抑制动动物附着。为了检验这一假设，我们设想了一个两步过程：1）对参与中肠刷状缘糖蛋白糖基化的蚊子中肠酶进行分子鉴定和生化表征，2）使用反向遗传学（RNAi基因沉默）来评估选定的中肠特异性糖基化酶对疟原虫感染的功能作用。这一系列实验将通过蚊子中肠生物学的基础糖生物学分析开辟新的研究途径。