Multi-modality Cellular Imaging of Vascular Inflammation

血管炎症的多模态细胞成像

• 批准号: 7118199
• 负责人: MICHAEL V MCCONNELL
• 金额: $ 39.12万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-22 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7118199
• 关键词: angiography; atherosclerotic plaque; bioimaging /biomedical imaging; bioluminescence; carotid artery; cell migration; genetic models; genetically modified animals; immune response; immunoregulation; inflammation; laboratory mouse; luciferin monooxygenase; macrophage; magnetic resonance imaging; model design /development; technology /technique development

DESCRIPTION (provided by applicant): Inflammation plays a critical role in the development and progression of vascular disease, including atherosclerosis. Strategies for visualizing the inflammatory process in vivo can help elucidate the timing of cellular infiltration, the signals that recruit inflammatory cells, and the role of these cells in the pathogenic process. A greater understanding of these mechanisms, through the application of sensitive imaging tools to this disease, will aid in the evaluation and development of effective therapies. The overall goals of this research are to develop and evaluate a sensitive and reliable platform for imaging vascular inflammation in relevant rodent models of disease and to use this technology to help understand the biological basis of atherosclerosis formation in order to guide novel approaches to therapy. Toward this end, we use two complementary imaging technologies - in vivo bioluminescence imaging and MRI. Bioluminescence has high sensitivity, is quantitative, and can reveal gene regulatory pathways. MRI provides high-resolution vascular imaging and tissue characterization and can image inflammatory cells using ultra-small superparamagnetic iron-oxide contrast agents (USPIOs). Specific Aims: 1) In vivo bioluminescence imaging of inflammatory cell trafficking to the vessel wall. 2) In vivo MRI of inflammatory cell trafficking to the vessel wall. 3) Bioluminescence and MRI of vascular inflammation in response to interventions. We have directed these studies to rodent models of vascular disease as they are ideal for studying the underlying molecular and cellular biology of disease progression and response to therapy. The long-term goal of our research is to improve the detection, evaluation, understanding, and treatment of human atherosclerotic disease. (End of Abstract)

描述（由申请人提供）： 炎症在包括动脉粥样硬化在内的血管疾病的发展和进展中起关键作用。可视化体内炎症过程的策略可以帮助阐明细胞浸润的时间，招募炎症细胞的信号以及这些细胞在致病过程中的作用。通过对这种疾病应用敏感的成像工具，更好地了解这些机制，将有助于评估和开发有效的治疗方法。本研究的总体目标是开发和评估一种敏感可靠的平台，用于在相关啮齿动物疾病模型中成像血管炎症，并使用该技术帮助了解动脉粥样硬化形成的生物学基础，以指导新的治疗方法。 为此，我们使用两种互补的成像技术-体内生物发光成像和MRI。生物发光具有灵敏度高、定量性强、可揭示基因调控途径等特点。MRI可提供高分辨率血管成像和组织表征，并可使用超小超顺磁性氧化铁造影剂（USPIO）对炎症细胞进行成像。 具体目的：1）炎症细胞向血管壁运输的体内生物发光成像。 2)炎症细胞向血管壁运输的体内MRI。 3)介入治疗后血管炎症的生物发光和MRI。 我们将这些研究导向了血管疾病的啮齿动物模型，因为它们是研究疾病进展和对治疗反应的基础分子和细胞生物学的理想模型。我们研究的长期目标是改善人类动脉粥样硬化疾病的检测、评估、理解和治疗。 (End摘要）