ApoE Receptor Pathways to Intraneuronal Abeta

ApoE 受体通往神经元内 Abeta 的途径

基本信息

  • 批准号:
    7081497
  • 负责人:
  • 金额:
    $ 31.28万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2006
  • 资助国家:
    美国
  • 起止时间:
    2006-05-01 至 2011-04-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Intraneuronal amyloid-p peptide (AP) accumulation is an early and toxic event in the pathogenesis of Alzheimer's disease (AD). Understanding cellular mechanisms that accelerate or inhibit intraneuronal A¿ accumulation may provide novel therapeutic strategies for AD. A¿ can accumulate inside neurons via receptor-mediated uptake. It can also accumulate via de novo processing of amyloid precursor protein (APR) to A¿ in the endocytic pathway. Our recent studies have shown that apolipoprotein E (apoE) receptors, members of the low-density lipoprotein receptor (LDLR) family, modulate A¿ uptake as well as APR endocytic trafficking and processing to A¿. In particular, we have demonstrated that LRP overexpression in the brain increases cell-associated A¿. A¿ can bind to apoE receptors either directly or indirectly via A¿ chaperones such as apoE. This proposal will focus on two apoE receptors, the LDLR-related protein (LRP) and LRP1B. These homologous receptors are both highly expressed in neurons and bind multiple ligands including A¿, apoE, and APP. However, evidence from our lab suggests that LRP and LRP1B play opposing roles in ligand endocytosis. While LRP mediates rapid endocytosis, LRP1B endocytoses very slowly and as a consequence, retains ligands at the cell surface. Our overall hypothesis is that LRP facilitates A¿ uptake, p production, and intraneuronal A¿ accumulation, and that LRP1B blocks these effects, thus inhibiting A¿ toxicity and pathogenesis of Alzheimer's disease. We have designed both in vivo and in vitro approaches to test our hypothesis. In Aim 1, we plan to determine the roles of LRP and LRP1B in intraneuronal accumulation in animal models. Because conventional LRP knockout is early embryonic lethal, our lab has generated conditional LRP forebrain-specific knockout mice. Together with the LRP1B knockout mice, we plan to test the roles of LRP and LRP1B in intraneuronal A¿ accumulation after brain A¿ infusion or after breeding with PDAPP amyloid model mice. In Aim 2, we will define the opposing roles of LRP and LRP1B in apoE-dependent and apoE-independent A¿ uptake in primary neurons. Impacts of altered LRP and/or LRP1B expression in neurons on A¿ uptake and intraneuronal accumulation will be assessed in the absence or presence of apoE. In Aim 3, we plan to dissect the mechanisms underlying the opposing roles of LRP and LRP1B in APP endocytic trafficking and A¿ production. Our proposed studies will take advantage of our experience in studying the cell biology of receptor-mediated endocytosis, APP endocytic trafficking, and A¿ metabolism using both in vitro and in vivo approaches. Because recent studies have established the causal role of intraneuronal A¿ in cognitive deficits prior to A¿ plaques, an understanding of the pathways leading to or protecting against intraneuronal AB accumulation may lead to specific targets for AD therapy.
描述(申请人提供):神经元内淀粉样蛋白-p肽(AP)蓄积是阿尔茨海默病(AD)发病机制中的早期毒性事件。了解加速或抑制神经元内A?积累的细胞机制可能为AD提供新的治疗策略。A可以通过受体介导的摄取在神经元内积累。它也可以通过内吞途径中淀粉样前体蛋白(APR)重新加工为A?而蓄积。我们最近的研究表明,载脂蛋白E(apoE)受体,低密度脂蛋白受体(LDLR)家族的成员,调节A <$摄取以及APR内吞运输和加工为A <$。特别是,我们已经证明,LRP在大脑中的过度表达增加了细胞相关的A?。A?可以直接或间接通过A?分子伴侣(如apoE)与apoE受体结合。这项提案将集中在两个apoE受体,低密度脂蛋白相关蛋白(LRP)和LRP 1B。这些同源受体都在神经元中高度表达,并结合多种配体,包括A?,apoE和APP。然而,我们实验室的证据表明,LRP和LRP 1B在配体内吞作用中发挥相反的作用。虽然LRP介导快速内吞作用,但LRP 1B内吞作用非常缓慢,因此将配体保留在细胞表面。我们的总体假设是,LRP促进A?摄取、p?产生和神经元内A?积累,LRP 1B阻断这些作用,从而抑制A?毒性和阿尔茨海默病的发病机制。我们设计了体内和体外方法来验证我们的假设。在目标1中,我们计划确定LRP和LRP 1B在动物模型中神经元内积聚中的作用。由于传统的LRP基因敲除是早期胚胎致死的,我们的实验室已经产生了条件性LRP前脑特异性敲除小鼠。与LRP 1B基因敲除小鼠一起,我们计划测试LRP和LRP 1B在脑A <$输注后或与PDAPP淀粉样蛋白模型小鼠繁殖后神经元内A <$积聚中的作用。在目标2中,我们将定义LRP和LRP 1B在原代神经元中apoE依赖性和apoE非依赖性A?摄取中的相反作用。将在存在或不存在apoE的情况下评估神经元中改变的LRP和/或LRP 1B表达对A?摄取和神经元内蓄积的影响。在目标3中,我们计划剖析LRP和LRP 1B在APP内吞运输和A?生产中的相反作用的机制。我们提出的研究将利用我们在研究受体介导的内吞作用,APP内吞运输和A?代谢的细胞生物学方面的经验,使用体外和体内方法。由于最近的研究已经确定了神经元内A?在A?斑块前认知缺陷中的因果作用,因此了解导致或保护神经元内AB积累的途径可能会导致AD治疗的特定靶点。

项目成果

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GUOJUN BU其他文献

GUOJUN BU的其他文献

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{{ truncateString('GUOJUN BU', 18)}}的其他基金

TREM2-mediated microglial dynamic function in Alzheimer disease
TREM2 介导的阿尔茨海默病小胶质细胞动态功能
  • 批准号:
    9914464
  • 财政年份:
    2020
  • 资助金额:
    $ 31.28万
  • 项目类别:
TREM2-mediated microglial dynamic function in Alzheimer disease
TREM2 介导的阿尔茨海默病小胶质细胞动态功能
  • 批准号:
    10088365
  • 财政年份:
    2020
  • 资助金额:
    $ 31.28万
  • 项目类别:
Integrative translational discovery of vascular risk factors in aging and dementia
衰老和痴呆血管危险因素的综合转化发现
  • 批准号:
    9421402
  • 财政年份:
    2017
  • 资助金额:
    $ 31.28万
  • 项目类别:
Integrative translational discovery of vascular risk factors in aging and dementia
衰老和痴呆血管危险因素的综合转化发现
  • 批准号:
    9001610
  • 财政年份:
    2015
  • 资助金额:
    $ 31.28万
  • 项目类别:
ApoE isoform-specific therapy for Alzheimer disease
ApoE 异构体特异性治疗阿尔茨海默病
  • 批准号:
    8744260
  • 财政年份:
    2013
  • 资助金额:
    $ 31.28万
  • 项目类别:
ApoE isoform-specific therapy for Alzheimer disease
ApoE 异构体特异性治疗阿尔茨海默病
  • 批准号:
    9104070
  • 财政年份:
    2013
  • 资助金额:
    $ 31.28万
  • 项目类别:
ApoE isoform-specific therapy for Alzheimer disease
ApoE 异构体特异性治疗阿尔茨海默病
  • 批准号:
    9291405
  • 财政年份:
    2013
  • 资助金额:
    $ 31.28万
  • 项目类别:
ApoE isoform-specific therapy for Alzheimer disease
ApoE 异构体特异性治疗阿尔茨海默病
  • 批准号:
    8894356
  • 财政年份:
    2013
  • 资助金额:
    $ 31.28万
  • 项目类别:
ApoE isoform-specific therapy for Alzheimer disease
ApoE 异构体特异性治疗阿尔茨海默病
  • 批准号:
    8608893
  • 财政年份:
    2013
  • 资助金额:
    $ 31.28万
  • 项目类别:
BRAIN LIPID METABOLISM, DENDRITES AND SYNAPSES IN AGING AND ALZHEIMER'S DISEASE
衰老和阿尔茨海默病中的脑脂质代谢、树突和突触
  • 批准号:
    8183828
  • 财政年份:
    2010
  • 资助金额:
    $ 31.28万
  • 项目类别:

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载脂蛋白E糖基化及其在阿尔茨海默病发病机制中的作用
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Impact of cysteine modifications of apolipoprotein E on the remnant lipoprotein metabolism
载脂蛋白E半胱氨酸修饰对残余脂蛋白代谢的影响
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