Federally Registered Human Embryonic Stem Cell Center

描述(由申请人提供)： 人类胚胎干细胞(HES)虽然是用于研究和临床的强大工具，但其可获得性有限。目前关于HES细胞的使用和限制获得联邦资金的资格的规定，使得合格队伍的分布和新调查人员的教育尽可能广泛，这是首要的。加州大学旧金山分校的HES分布和培训计划是为了向世界各地学术机构的研究人员提供HSF-6(UCO6)和HSF-1(UCO1)，这两个HES细胞系是UCSF在NIH人类胚胎干细胞注册登记中衍生的。最初计划的目标是分发来自加州大学旧金山分校的两个HES细胞系，HSF-6和HSF-1，并继续和扩大访问调查人员的培训计划。已经建立了分配计划，在该计划下，细胞大量扩增，并对无菌、HES细胞特性和分化潜力进行测试。已经启动了一项针对合格技术人员的培训计划，为受援实验室提供在其自己的实验室成功建立HES培养所需的专业知识。最后，加州大学旧金山分校现有的HES细胞系已经适应了良好的实验室实践。这项提案延续并扩大了该方案于2002年5月开始的努力。分发计划将扩大到包括有用的转基因HES系、GFP标记的细胞和来自HSF-6系的Tet可诱导细胞。协议书和培训手册将进行扩展，以包含我们实验室中使用的全套HES细胞繁殖和表征协议。为了帮助向世界各地的学术机构分发信息，该计划的网站将扩大，为用户和感兴趣的个人提供关于细胞系和HES研究的信息。此外，还将公布这些协议。最后，HSF-6和HSF-1株将被转移到加州大学旧金山分校的GMP设施，以创建一个临床使用的认证细胞库。因此，该计划的扩展将支持HES细胞在研究和临床社区中的实用。

DESCRIPTION (provided by applicant): Human embryonic stem (hES) cells, although powerful tools for research and clinical uses, are limited in availability. Current regulations regarding the use of hES cells and limiting eligibility for federal funding, makes the distribution of the eligible lines and education of new investigators as widely as possible, of primary importance. The UCSF hES distribution and training programs were initiated in response to these limitations to supply investigators in academic institutions throughout the world with HSF-6 (UCO6) and HSF-1 (UCO1), the two hES cell lines derived at UCSF on The NIH Registry of Human Embryonic Stem Cells. The goal of the original program was to distribute the two hES cell lines derived at UCSF, HSF-6 and HSF-1, and to continue and expand the training program for visiting investigators. A distribution program has been established, under which the cells are expanded in large lots, and tested for sterility, hES cell characteristics, and differentiation potential. A training program has been initiated for qualified technical staff to provide recipient labs with the expertise necessary to successfully establish hES cultures in their own lab. Finally, the hES cell lines available at UCSF, have been adapted to good laboratory practices. This proposal extends and expands the efforts begun by this program in May of 2002. The distribution program will be expanded to include useful genetically modified hES lines, GFP-marked cells and Tet-inducible cells derived from the HSF-6 line. The protocol book and training manual will be expanded to contain the full set of protocols for hES cell propagation and characterization utilized in our lab. To assist in the distribution to academic facilities throughout the world, the program website will be expanded the provide users and interested individuals with information about the cell lines and hES research. In addition, these protocols will be published. Finally, the HSF-6 and HSF-1 lines will be transferred to the UCSF GMP facility to create a bank of certified cells for clinical use. Thus, the extension of this program will support the utility of hES cells for both the research and clinical communities.