Human Endometrial Tissue and DNA Bank

项目总结(见说明)： 我们在加州大学旧金山分校的NIH U54 SCCPIR中心的这次更新提出了一种跨学科的团队科学方法，以阐明早期发育的精液事件潜在的机制，因为它们与人类不孕不育的起源和生物学后果有关。我们的调查人员 专注于从卵母细胞能力到胚胎和胎盘发育到植入的发展连续体，并促进在生殖研究、先进技术、信息学数据分析、指导和社区参与方面的卓越和创新的共同愿景。我们结合了强大的实验技术、系统方法和计算分析来解决关键问题 干细胞生物学、表观基因组学和临床生殖医学的交叉。为了实现这些目标，我们提出了四个由现有调查人员高度互动的项目和一个由三个核心支持的新调查人员的试点项目。项目I(马尔科·孔蒂)的重点是机制 这些项目包括：项目II(Susan Fisher)将调查滋养层细胞分化早期阶段的分子分析；项目III(Robert Blelloch)将重点研究滋养层细胞分化的转录后调控；项目IV(Linda Giudice)将调查人类胚胎着床的子宫内膜发育；以及试点项目(NAMTan)将调查子宫内膜异位症的发病和病理生理机制，子宫内膜异位症是一种在女性中常见的疾病，与不孕症和不良妊娠结局有关。这些项目得到核心A管理(Linda Giudice)、计算生物学研究核心B(Jun Song)和教育与社区核心C(外联Synthia Mellon)的支持。我们的长期合作 目标是从根本上了解正常和异常早期发育的机制，并开发诊断、预防和治疗不孕不育的新策略；优化生育和怀孕结果并将不良后果降至最低；教育有抱负的学生、公众、卫生保健专业人员和患者关于人类发育和与异常发育相关的疾病；以及启发 职业选择和培育是生殖健康这一重要领域的职业发展和研究。 这些目标与NICHD的使命是一致的。

PROJECT SUMMARY (See Instructions): This renewal of our NIH U54 SCCPIR Center at the University of California, San Francisco proposes a transdisciplinary, team science approach to elucidate mechanisms underlying seminal events in early development as they relate to the origins and biological consequences of human infertility. Our investigators focus on the developmental continuum from oocyte competence to embryo and placental development to implantation and promote a shared vision of excellence and innovation in reproductive research, advanced technologies, informatics data analyses, mentoring, and community engagement. We combine powerful experimental techniques, system approaches and computational analyses to address critical questions at the intersection of stem cell biology, epigenomics, and clinical reproductive medicine. To accomplish these goals, we have proposed four highly interactive projects by established investigators and one pilot project with a new investigator, which are supported by three cores. Project I (Marco Conti) focuses on mechanisms controlling oocyte developmental competence and early embryo development; Project II (Susan Fisher) will investigate a molecular analysis of the early stages of trophoblast differentiation; Project III (Robert Blelloch) focuses on post-transcriptional regulation of trophoblast differentiation; Project IV (Linda Giudice) will investigate development of human endometrium for embryonic implantation; and the Pilot Project (Nam Tran) proposes to investigate molecular mechanisms underlying the pathogenesis and pathophysiology of endometriosis, a common disorder in women associated with infertility and poor pregnancy outcomes These projects are supported by Core A for administration (Linda Giudice), Core B for computational biology research (Jun Song), and Core C for education and community outreach (Synthia Mellon). Our long-term goals are to understand mechanisms underlying normal and abnormal early development fundamentally and for developing novel strategies to diagnose, prevent and treat infertility; optimize fertility and pregnancy outcomes and minimize adverse outcomes; educate aspiring students, the public, health care professionals and patients about human development and disorders associated with abnormal development; and inspire career choice and nurture career development in this important area of reproductive health and research. These goals are consistent with the mission of the NICHD.