MHC Typing of Macaques used in AIDS Research

描述（由申请人提供）： 在HIV疫苗开发和发病机制研究中，各种猕猴作为动物模型的实用性越来越高，需要继续参考这些物种的MHC分型实验室。我们计划继续为北美和欧洲科学界提供猕猴MHC分型服务。最初，这将包括对编码MHC I类和II类分子的等位基因进行PCR-SSP测试，这些分子结合来自SIV和SHIV的肽。我们正在开发用于分析印度恒河猴、中国恒河猴和食蟹猴MHC I类和II类等位基因的其他分子技术。此外，我们将继续为希望在自己的实验室建立MHC分型的个别实验室提供培训。最后，我们将继续开发一组特征良好的细胞系，这将是非常宝贵的猕猴MHC的分析。 到目前为止，我们已经为大约30名研究人员使用27，069次测试对3，800多只猕猴进行了分型。这些打字工作支持了50多份手稿的出版。因此，这项建议的两个具体目标是： I.为从事猕猴（印度恒河猴、中国恒河猴和食蟹猴）研究的研究人员提供I类和II类基因座的MHC分型。 二.使HLA分型技术适用于猕猴（印度恒河猴、中国恒河猴和食蟹猴）I类和II类的分子分型。

DESCRIPTION (provided by applicant): The increased utility of various species of macaques as animal models in both HIV vaccine development and pathogenesis studies necessitates the continuation of reference MHC typing laboratories for these species. We plan to continue to offer services to both the North American and European scientific community for MHC typing of macaques. Initially, this will include PCR-SSP tests for alleles encoding MHC class I and II molecules that bind peptides derived from SIV and SHIV. We are developing additional molecular techniques for analysis of the Indian rhesus and Chinese rhesus and Cynomolgus macaque MHC class I and class II alleles. Additionally, we will continue to offer training for individual laboratories that wish to set up MHC typing in their own laboratories. Finally, we will continue to develop a panel of well-characterized cell lines that will be invaluable for the analysis of the MHC in the macaque. To date we have typed more than 3,800 macaques for approximately 30 investigators using 27,069 tests. These typings have supported the publication of over 50 manuscripts. The two specific aims of this proposal are, therefore, to: I. Offer MHC typing of the class I and II loci for investigators working with macaques (Indian rhesus, Chinese rhesus and Cynomolgus). II. Adapt technologies for HLA Typing to molecular typing of macaque (Indian rhesus, Chinese rhesus and Cynomolgus) class I and II.