Acquisition of Peptide Synthesizer for Core Facility

描述（由申请人提供）：在当前蓬勃发展的蛋白质组学和基因组学研究的时代，校园研究人员对定制肽的需求正在以很大的速度扩大。资金要求购买一个国家的最先进的肽合成仪和组合肽合成仪，以满足一大群NIH资助的研究人员在威斯康星大学麦迪逊分校的合成需求。该设备将被安置在现有的肽合成核心设施的威斯康星州大学生物技术中心。一个全职博士水平的设施经理监督这个设施。此外，还有两名全职支助人员和两名学生小时工。该设施见证了校园对定制肽的需求显着增加。合成的肽的数量从1996年的183种肽稳定增长到2003年的一千多种。在过去的12个月里，核心设施已经为代表25个不同部门的大约50个校园研究小组生产了肽。目前的需求已经超过了该设施的能力，一些现有设备的使用寿命即将结束。 除了由核心实验室人员在成本回收的基础上进行完全定制的合成外，肽核心实验室还提供独特的“自己动手”肽合成程序。根据这一方案，主要和次要用户实验室的研究生接受核心设施人员的培训，以操作合成仪，并以较低的成本在核心实验室进行自己的合成。

DESCRIPTION (provided by applicant): In the current era of burgeoning proteomics and genomics efforts, the needs of campus researchers for custom peptides is expanding at a great rate. Funds are requested to purchase a state-of-the-art peptide synthesizer and a combinatorial peptide synthesizer to meet the synthetic needs of a large group of NIH funded researchers at UW-Madison. The equipment would be housed in the existing Peptide Synthesis core facility of the University of Wisconsin Biotechnology Center. A full-time PhD level Facility Manager oversees this facility. In addition, there are two full-time support staff and two student hourly employees. The facility has witnessed a remarkable increase in the campus demand for custom peptides. The number of peptides synthesized has grown steadily from 183 peptides in 1996 to over a thousand in the year 2003. Over the past 12 months the core facility has made peptides for approximately 50 campus research groups, representing 25 different departments. Current demand already exceeds the capacity of the facility and some of the existing equipment is nearing the end of its useful life. In addition to full custom synthesis carried out by core lab personnel on a cost recovery basis, the peptide core lab also offers a unique program of "do-it-yourself" peptide synthesis. Under this program, graduate students in the laboratories of the primary and secondary users are trained by the core facility personnel to operate the synthesizers and carry out their own synthesis in the core lab at reduced cost.