Acquisition of Peptide Synthesizer for Core Facility

描述(申请人提供)：在当前蛋白质组学和基因组学努力蓬勃发展的时代，校园研究人员对定制多肽的需求正在以极大的速度扩大。需要资金购买最先进的多肽合成器和组合多肽合成器，以满足密歇根大学麦迪逊分校NIH资助的一大批研究人员的合成需求。这些设备将安置在威斯康星大学生物技术中心现有的多肽合成核心设施中。一名全职博士级别的设施经理负责监督该设施。此外，还有两名全职支持人员和两名学生小时工。该设施见证了校园对定制多肽的需求显著增加。合成的多肽数量稳步增长，从1996年的183个增加到2003年的1000多个。在过去的12个月里，核心设施已经为大约50个校园研究小组制造了多肽，代表了25个不同的部门。目前的需求已经超过了该设施的能力，一些现有设备的使用寿命即将结束。 除了由核心实验室人员在收回成本的基础上进行完全定制的合成外，多肽核心实验室还提供独特的“自己动手”的多肽合成计划。根据这一计划，一次和二次用户实验室的研究生由核心设施人员进行培训，以操作合成器并在核心实验室以较低的成本进行自己的合成。

DESCRIPTION (provided by applicant): In the current era of burgeoning proteomics and genomics efforts, the needs of campus researchers for custom peptides is expanding at a great rate. Funds are requested to purchase a state-of-the-art peptide synthesizer and a combinatorial peptide synthesizer to meet the synthetic needs of a large group of NIH funded researchers at UW-Madison. The equipment would be housed in the existing Peptide Synthesis core facility of the University of Wisconsin Biotechnology Center. A full-time PhD level Facility Manager oversees this facility. In addition, there are two full-time support staff and two student hourly employees. The facility has witnessed a remarkable increase in the campus demand for custom peptides. The number of peptides synthesized has grown steadily from 183 peptides in 1996 to over a thousand in the year 2003. Over the past 12 months the core facility has made peptides for approximately 50 campus research groups, representing 25 different departments. Current demand already exceeds the capacity of the facility and some of the existing equipment is nearing the end of its useful life. In addition to full custom synthesis carried out by core lab personnel on a cost recovery basis, the peptide core lab also offers a unique program of "do-it-yourself" peptide synthesis. Under this program, graduate students in the laboratories of the primary and secondary users are trained by the core facility personnel to operate the synthesizers and carry out their own synthesis in the core lab at reduced cost.