Acquisition of Peptide Synthesizer for Core Facility

描述（由申请人提供）：在当前蛋白质组学和基因组学蓬勃发展的时代，校园研究人员对定制肽的需求正在以极大的速度增长。资金要求购买最先进的肽合成器和组合肽合成器，以满足一大批NIH资助的威斯康星大学麦迪逊分校研究人员的合成需求。该设备将被安置在威斯康星大学生物技术中心现有的肽合成核心设施中。一个全职的博士级别的设施经理监督这个设施。此外，还有两名全职支持人员和两名学生小时工。该设施见证了校园定制肽需求的显著增长。合成的多肽数量从1996年的183条稳步增长到2003年的1000多条。在过去的12个月里，核心设施为代表25个不同部门的大约50个校园研究小组制造了多肽。目前的需求已超过该设施的能力，一些现有设备已接近其使用寿命。

DESCRIPTION (provided by applicant): In the current era of burgeoning proteomics and genomics efforts, the needs of campus researchers for custom peptides is expanding at a great rate. Funds are requested to purchase a state-of-the-art peptide synthesizer and a combinatorial peptide synthesizer to meet the synthetic needs of a large group of NIH funded researchers at UW-Madison. The equipment would be housed in the existing Peptide Synthesis core facility of the University of Wisconsin Biotechnology Center. A full-time PhD level Facility Manager oversees this facility. In addition, there are two full-time support staff and two student hourly employees. The facility has witnessed a remarkable increase in the campus demand for custom peptides. The number of peptides synthesized has grown steadily from 183 peptides in 1996 to over a thousand in the year 2003. Over the past 12 months the core facility has made peptides for approximately 50 campus research groups, representing 25 different departments. Current demand already exceeds the capacity of the facility and some of the existing equipment is nearing the end of its useful life. In addition to full custom synthesis carried out by core lab personnel on a cost recovery basis, the peptide core lab also offers a unique program of "do-it-yourself" peptide synthesis. Under this program, graduate students in the laboratories of the primary and secondary users are trained by the core facility personnel to operate the synthesizers and carry out their own synthesis in the core lab at reduced cost.