MALDI TOF/TOF Mass Spectrometer

描述(由申请人提供)：我们建议购买一台MALDI TOF/TOF质谱仪，这将为威斯康星大学麦迪逊分校的健康相关研究提供关键支持。将受益的具体研究项目包括：1)设计非天然低聚物(叶状体)以阻断与疾病相关的蛋白质-蛋白质相互作用；2)开发用作组织工程基质或涂层的生物材料，用于植入式整形医疗设备；3)鉴定线虫中的神经肽，这是神经系统功能的简单模型；4)设计干扰细菌群体感应的小分子，作为基础生物学研究的工具，并导致新的抗菌剂；5)绘制水果和其他食品中发现的多酚(单宁)与细胞培养和体外炎症、细菌黏附和氧化模型的生物活性之间的关系；6)阐明伴随核糖体生物合成而发生的蛋白质折叠；7)设计新的聚合物，以模拟宿主防御肽的选择性抗菌活性。拟议的仪器将是对华盛顿大学化学部的MS设施的重要补充，因为它将允许通过裂解进行常规结构确定(例如，多肽、多肽类似物和聚合物的测序)。化学MS设施在威斯康星州大学校园中是独一无二的，它允许学生和其他研究人员24小时接触，这对于上述项目的快速进展至关重要。因此，所请求的仪器提供的能力将构成对美国一家主要研究机构的科学基础设施的重要补充。

DESCRIPTION (provided by applicant): We propose to acquire a MALDI TOF/TOF mass spectrometer, which will provide crucial support for health-related research at UW-Madison. Specific research projects that will benefit include: 1) the design of unnatural oligomers (foldamers) to block disease-associated protein-protein interactions; 2) the development of biomaterials that function as tissue engineering substrates or coatings for implanted orthopedic medical devices; 3) the identification of neuropeptides in the nematode, a simple model for nervous system function; 4) the design of small molecules that disrupt bacterial quorum sensing, which can serve as tools for basic biological research and as leads to new antibacterial agents; 5) the mapping of relationships between polyphenols (tannins) found in fruits and other foods and bioactivity in cell culture and in vitro models of inflammation, bacterial adherence and oxidation; 6) the elucidation of protein folding that occurs concomitantly with ribosomal biosynthesis; 7) the design of new polymers that mimic the selective antimicrobial activity of host-defense peptides. The proposed instrument will be a crucial addition to the MS facility in the UW Chemistry Department because it will allow routine structure determination via fragmentation (e.g., sequencing of peptides, peptide analogues and polymers). The Chemistry MS facility is unique on the UW campus in allowing 24-hour access to students and other researchers, which is essential for rapid progress in the projects described above. Therefore, the capabilities provided by the requested instrument would constitute an important addition to the scientific infrastructure at a major US research institution.