Understanding the role of N, a TIR-NB-LRR protein, in plant innate immunity

了解 TIR-NB-LRR 蛋白 N 在植物先天免疫中的作用

• 批准号: 7144788
• 负责人: SAVITHRAMMA P DINESH-KUMAR
• 金额: $ 29.67万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7144788
• 关键词: Arabidopsis; affinity chromatography; antiviral agents; apoptosis; biological signal transduction; chromatin immunoprecipitation; gene mutation; immune response; immunity; microarray technology; molecular site; nucleic acid sequence; plant diseases; plant genetics; plant physiology; plant proteins; protein localization; protein structure function; replicase; tobacco mosaic virus; transcription factor; yeast two hybrid system

DESCRIPTION (provided by applicant): Our goal is to understand the molecular mechanisms by which plant resistance (R) proteins mediate innate immune signaling. We will continue to use the interaction between the N resistance gene and tobacco mosaic virus (TMV) as a model system. The N protein is a member of the TIR-NB-LRR family of R proteins and is hypothesized to specifically recognize the 50 kDa helicase domain (TMV-p50) of the TMV replicase protein to trigger induction of innate immune responses. We will use a combination of genetic, molecular, biochemical and cellular approaches to understand the function of N in innate immunity. We will determine how N recognizes TMV-p50. Recent evidence from our laboratory indicates that N and TMV-p50 associate in intact, living tissue in a narrow period over the course of the host-pathogen interaction and resistance. However, this interaction is not direct and may be dependent on an N-TIR interacting protein we call NTI1. This suggests that the TIR domain might be involved in TMV recognition. We will therefore determine domain(s) of N responsible for recognition of TMV-p50. Further, we will characterize the role of NTI1 in mediating the N and TMV-p50 association. To characterize TMV-p50 recognition complex, we will isolate and identify components of N and NTH protein complexes. Recent results from our laboratory indicate that N associates directly with a transcription factor we call TFN5. Thus, N, in addition to being involved in pathogen recognition, may also directly control downstream transcriptional events. We will determine, in vivo, the interaction dynamics of N and TFN5 before and after TMV infection. We will identify and characterize TFN5 targets during the innate immune response. An increasing world population, from 6 to 12 billion, in the next 50 years will create greater demand for food, fiber, fuel and Pharmaceuticals. We therefore need increased knowledge of plant genes in order to manipulate them to engineer improved crops. Worldwide annual losses of crop productivity due to diseases are valued over $100 billion. Therefore, control of pathogen-induced diseases using natural R genes and other cellular genes that function in the resistance signaling pathways may provide tremendous benefits. This manipulation of endogenous genes will have significant positive impact on poorer citizens. It will serve the environment by offering an alternative to pesticide use to prevent diseases and thus health risks.

描述（由申请人提供）：我们的目标是了解植物抗性（R）蛋白介导先天免疫信号传导的分子机制。我们将继续使用N抗性基因和烟草花叶病毒（TMV）之间的相互作用作为一个模式系统。N蛋白是R蛋白的TIR-NB-LRR家族的成员，并且被假设特异性识别TMV复制酶蛋白的50 kDa解旋酶结构域（TMV-p50）以触发先天免疫应答的诱导。我们将使用遗传学，分子，生物化学和细胞方法的组合来了解N在先天免疫中的功能。我们将确定N如何识别TMV-p50。我们实验室的最新证据表明，N和TMV-p50在完整的活组织中在宿主-病原体相互作用和抗性的过程中在一个狭窄的时期内相关联。然而，这种相互作用不是直接的，可能取决于我们称为NTI 1的N-TIR相互作用蛋白。这表明TIR结构域可能参与TMV的识别。因此，我们将确定负责识别TMV-p50的N的结构域。此外，我们将表征NTI 1在介导N和TMV-p50关联中的作用。为了表征TMV-p50识别复合物，我们将分离和鉴定N和NTH蛋白复合物的组分。我们实验室最近的结果表明，N与我们称为TFN 5的转录因子直接相关。因此，N除了参与病原体识别之外，还可以直接控制下游转录事件。我们将在体内确定N和TFN 5在TMV感染前后的相互作用动力学。我们将在先天免疫应答期间鉴定和表征TFN 5靶点。未来50年，世界人口将从60亿增加到120亿，这将对粮食、纤维、燃料和药品产生更大的需求。因此，我们需要增加对植物基因的了解，以便操纵它们来设计改良作物。全世界每年因疾病造成的作物生产力损失价值超过1000亿美元。因此，使用天然R基因和在抗性信号传导途径中起作用的其他细胞基因来控制病原体诱导的疾病可以提供巨大的益处。这种对内源基因的操纵将对较贫穷的公民产生重大的积极影响。它将为环境服务，提供一种替代杀虫剂的方法，以预防疾病，从而预防健康风险。