Acquisition of Zeiss LSM 510 META confocal microscope

购买 Zeiss LSM 510 META 共焦显微镜

• 批准号: 7220785
• 负责人: SAVITHRAMMA P DINESH-KUMAR
• 金额: $ 45.53万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7220785
• 关键词: Animal Model; Arts; Biological Process; Biology; Cell Maintenance; Cell division; Cells; Chemicals; Complex; Cytoskeleton; DNA-Protein Interaction; Defect; Development; Drosophila genus; Fluorescence Microscopy; Funding; Future; Image Analysis; Imaging Techniques; Impaired cognition; Investigation; Lasers; Learning; Life; Light; Light Microscope; Mediating; Memory; Microscope; Molecular; Molecular Motors; Mus; Natural Immunity; Neural Crest; Neurons; Plants; Principal Investigator; Proteins; Proteolysis; Research; Research Activity; Research Personnel; Scanning; Science; Side; Spinal; Stem cells; System; Tissues; Ubiquitin; United States National Institutes of Health; Universities; Urination; Zebrafish; cell motility; instrument; instrumentation; multicatalytic endopeptidase complex; neurogenesis; optical imaging; programs; somitogenesis; synaptogenesis

DESCRIPTION (provided by applicant): We are requesting funds for the acquisition of a Zeiss LSM 510 META confocal microscope. Acquisition of the requested state-of-the-art instrument would allow users of this proposal to perform live cell and tissue imaging analyses. The proposed laser scanning confocal microscope will be a major upgrade to our existing light microscope facility on the Science Hill campus of Yale University. Principal users designated in this proposal are already using optical imaging techniques to answer a variety of basic, fundamental questions in biology. The requested instrument will enhance their ability to perform complex and cutting edge image analysis required by many of the modern model organisms like zebrafish, Drosophila, plants and mice. Research activities facilitated by this instrument will include investigation of innate immunity (Dinesh-Kumar, Principal Investigator), cytoskeleton and molecular motors (Mark Mooseker, co-investigator), Drosophila olfactory system (John Carlson), light regulated developmental switch (Xing-Wang Deng), origin and induction of neural crest stem cells (Martin Garcia-Castro), ubiquitin/proteasome-mediated targeted proteolysis (Mark Hochstrasser), zebrafish somitogenesis (Scott Holley), cellular and molecular mechanisms of synaptogenesis (Haig Keshishian), cytoskeletal dynamics and cellular motility (Thomas Pollard), chemical biology of protein-protein and protein-DNA interactions inside the cell (Alanna Schepartz), axonal, dendritic and spinal abnormalities leading to neuronal developmental defects and cognitive impairment (Elke Stein), learning and memory (David Wells), and asymmetric cell division in neurogenesis and stem-cell maintenance (Weimin Zhong). The requested state-of-the-art instrument undoubtedly will have a significant impact on the research programs on the Science Hill side of the Yale campus by filling a substantial void in our available instrumentation. Given the current and future centrality of fluorescence microscopy in studying the dynamics and complexity of biological processes, access to state-of-the-art instrument will maximize the impact of our currently funded NIH research.

描述（由申请人提供）：我们正在申请资金用于购买蔡司LSM 510 META共聚焦显微镜。获得所要求的最先进的仪器将允许本提案的用户进行活细胞和组织成像分析。拟议中的激光扫描共聚焦显微镜将是对耶鲁大学科学山校区现有光学显微镜设备的重大升级。本提案中指定的主要用户已经在使用光学成像技术来回答生物学中的各种基本问题。所要求的仪器将提高他们执行复杂和尖端图像分析的能力，许多现代模式生物，如斑马鱼，果蝇，植物和小鼠需要。该仪器将用于研究先天免疫（首席研究员dinish - kumar）、细胞骨架和分子马达（联合研究员Mark Mooseker）、果蝇嗅觉系统（John Carlson）、光调节发育开关（sing - wang Deng）、神经嵴干细胞的起源和诱导（Martin Garcia-Castro）、泛素/蛋白酶体介导的靶向蛋白水解（Mark Hochstrasser）、斑马鱼体细胞发生（Scott Holley）、突触发生的细胞和分子机制（Haig Keshishian），细胞骨架动力学和细胞运动（Thomas Pollard），细胞内蛋白质-蛋白质和蛋白质- dna相互作用的化学生物学（Alanna Schepartz），导致神经元发育缺陷和认知障碍的轴突、树突和脊柱异常（Elke Stein），学习和记忆（David Wells），以及神经发生和干细胞维持中的不对称细胞分裂（钟伟民）。所要求的最先进的仪器无疑将对耶鲁大学科学山校区的研究项目产生重大影响，填补了我们现有仪器的巨大空白。鉴于荧光显微镜在研究生物过程的动态和复杂性方面的当前和未来的中心地位，获得最先进的仪器将最大限度地影响我们目前资助的NIH研究。