Mineral-Matrix Relations in Calcifying Tissues
Mineral-Matrix Relations in Calcifying Tissues
批准号:
7053352
负责人:
WILLIAM J LANDIS
金额:
$30.83万
依托单位国家:
美国
项目类别:
财政年份:
1992
资助国家:
美国
项目状态:
已结题
起止时间:
1992-05-01 至 2009-03-31
关键词:
athymic mouseatomic force microscopybone sialoproteincalcificationcollagenextracellular matrix proteinsin situ hybridizationinfrared spectrometryintermolecular interactionlaboratory mouselaser capture microdissectionlimb bonemineralsmolecular dynamicsnormal ossificationosteocalcinosteopontintendonstissue /cell culturetissue engineeringtransmission electron microscopyturkeys
中文摘要
描述(由申请人提供):本提案的主要目标是
英文摘要
DESCRIPTION (provided by applicant): The major goal of this proposal is to
obtain new information concerning the process of vertebrate skeletal
mineralization at the molecular and cellular level. An understanding of
mineralization is significant because it is the foundation for mechanical
support of the body, for skeletal adaptation under mechanical constraint, as an
ion reservoir, and for treating, preventing, and curing a variety of skeletal
abnormalities that affect human well being. The proposed study will focus on
three model systems, the calcifying tendon from normal turkeys, long bone from
exercised mice, and a tissue-engineered model of phalanges and small joints.
These systems will be examined under natural conditions and after changes that
alter mechanical forces acting on them. A combination of molecular biological,
biochemical, immunochemical, and structural techniques will be used to
determine the processes by which cells elaborate an organic matrix that
mineralizes, as well as the chemical and structural nature of matrix-mineral
interaction. The specific hypothesis to be examined is: the structural and
functional adaptation of a calcifying tissue to mechanical perturbation is
directed by expression of osteopontin (opn), osteocalcin (oc) and bone
sialoprotein (bsp), and their interactions with mineral deposition. The
Specific Aims that will probe this structure-function relationship are to (1)
establish the expression pattern of the selected genes (opn, oc, bsp) as
compared to specific matrix proteins, matrix receptors, cytoskeletal proteins
and mineral formation in the three model systems; (2) determine the adaptive
response at the tissue level in terms of these parameters following
perturbation of the tendon model altered by a surgically-induced mechanical
change, and of the bone model through exercise; and (3) assess the adaptive
response at the organ level by determining the pattern of protein expression
and protein-mineral interactions in the phalanx-joint model placed under a
muscular load. This multifaceted approach will contribute to an increased
understanding of the chemistry, structure, organization, and interactions of
relevant molecules and cells that are critically important for the function of
mineralizing tissues and their adaptation under mechanical loading.
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Analysis of human osteoarthritic connective tissue by laser capture microdissection and QRT-PCR.
通过激光捕获显微切割和 QRT-PCR 分析人类骨关节炎结缔组织。
DOI:
10.1080/03008200701692685
发表时间:
2007
期刊:
Connective tissue research
影响因子:
2.9
作者:
[Scharschmidt,Thomas, Jacquet,Robin, Laskovski,Jovan, Lowder,Elizabeth, Weiner,Scott, Landis,WilliamJ]
通讯作者:
Landis,WilliamJ
Tissue-engineered meniscal constructs.
组织工程半月板结构。
DOI:
--
发表时间:
2007
期刊:
American journal of orthopedics (Belle Mead, N.J.)
影响因子:
--
作者:
[Schoenfeld,AndrewJ, Landis,WilliamJ, Kay,DavidB]
通讯作者:
Kay,DavidB
DOI:
--
发表时间:
1991
期刊:
Journal of computer-assisted microscopy
影响因子:
--
作者:
[B. McEwen;M. J. Song;W. Landis]
通讯作者:
B. McEwen;M. J. Song;W. Landis
Mineral characterization in calcifying tissues: atomic, molecular and macromolecular perspectives.
钙化组织中的矿物质特征:原子、分子和大分子视角。
DOI:
10.3109/03008209609005267
发表时间:
1996
期刊:
Connective tissue research
影响因子:
2.9
作者:
[Landis,WJ]
通讯作者:
Landis,WJ
Modified aminosilane substrates to evaluate osteoblast attachment, growth, and gene expression in vitro.
修饰氨基硅烷底物,用于评估体外成骨细胞附着、生长和基因表达。
DOI:
10.1002/jbm.a.30731
发表时间:
2006
期刊:
Journal of biomedical materials research. Part A
影响因子:
--
作者:
[Siperko,LM, Jacquet,R, Landis,WJ]
通讯作者:
Landis,WJ
共 17 条
STUDY OF THE FORMATION OF ENAMEL CRYSTALLITES BY AMELOGENINS
-
批准号:7598369
-
项目类别:
-
资助金额:$0.23万
-
财政年份:2007
-
负责人:WILLIAM J LANDIS
-
依托单位:
STUDY OF THE FORMATION OF ENAMEL CRYSTALLITES BY AMELOGENINS
-
批准号:7357291
-
项目类别:
-
资助金额:$0.23万
-
财政年份:2006
-
负责人:WILLIAM J LANDIS
-
依托单位:
8th Conference - Chemistry & Biology Mineralized Tissue
-
批准号:6711598
-
项目类别:
-
资助金额:$3.5万
-
财政年份:2004
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:2080716
-
项目类别:
-
资助金额:$29.15万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
Mineral-Matrix Relations in Calcifying Tissues
-
批准号:6625728
-
项目类别:
-
资助金额:$34.31万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
Mineral-Matrix Relations in Calcifying Tissues
-
批准号:6879176
-
项目类别:
-
资助金额:$31.57万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:2080715
-
项目类别:
-
资助金额:$28.29万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL-MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:2700217
-
项目类别:
-
资助金额:$7.55万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL-MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:2413975
-
项目类别:
-
资助金额:$35.7万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:2876555
-
项目类别:
-
资助金额:$29.54万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:2909793
-
项目类别:
-
资助金额:$34.52万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL-MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:3161898
-
项目类别:
-
资助金额:$33.35万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL-MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:2080717
-
项目类别:
-
资助金额:$34.44万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
MINERAL-MATRIX RELATIONS IN CALCIFYING TISSUES
-
批准号:3161897
-
项目类别:
-
资助金额:$34.56万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
Mineral-Matrix Relations in Calcifying Tissues
-
批准号:6478399
-
项目类别:
-
资助金额:$37.62万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
Mineral-Matrix Relations in Calcifying Tissues
-
批准号:6726070
-
项目类别:
-
资助金额:$34.31万
-
财政年份:1992
-
负责人:WILLIAM J LANDIS
-
依托单位:
GORDON RESEARCH CONFERENCE ON CALCIUM PHOSPHATES, 1987
-
批准号:3433716
-
项目类别:
-
资助金额:$0.55万
-
财政年份:1987
-
负责人:WILLIAM J LANDIS
-
依托单位:
CORE--ELECTRON MICROSCOPY
-
批准号:4689752
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:WILLIAM J LANDIS
-
依托单位:
ELECTRON OPTICAL AND MICROPROBE ANALYSIS OF BONE AND OTHER MINERALIZED TISSUES
-
批准号:4689748
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:WILLIAM J LANDIS
-
依托单位:
海外基金