Mineral-Matrix Relations in Calcifying Tissues
钙化组织中的矿物质-基质关系
基本信息
- 批准号:7053352
- 负责人:
- 金额:$ 30.83万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1992
- 资助国家:美国
- 起止时间:1992-05-01 至 2009-03-31
- 项目状态:已结题
- 来源:
- 关键词:athymic mouseatomic force microscopybone sialoproteincalcificationcollagenextracellular matrix proteinsin situ hybridizationinfrared spectrometryintermolecular interactionlaboratory mouselaser capture microdissectionlimb bonemineralsmolecular dynamicsnormal ossificationosteocalcinosteopontintendonstissue /cell culturetissue engineeringtransmission electron microscopyturkeys
项目摘要
DESCRIPTION (provided by applicant): The major goal of this proposal is to
obtain new information concerning the process of vertebrate skeletal
mineralization at the molecular and cellular level. An understanding of
mineralization is significant because it is the foundation for mechanical
support of the body, for skeletal adaptation under mechanical constraint, as an
ion reservoir, and for treating, preventing, and curing a variety of skeletal
abnormalities that affect human well being. The proposed study will focus on
three model systems, the calcifying tendon from normal turkeys, long bone from
exercised mice, and a tissue-engineered model of phalanges and small joints.
These systems will be examined under natural conditions and after changes that
alter mechanical forces acting on them. A combination of molecular biological,
biochemical, immunochemical, and structural techniques will be used to
determine the processes by which cells elaborate an organic matrix that
mineralizes, as well as the chemical and structural nature of matrix-mineral
interaction. The specific hypothesis to be examined is: the structural and
functional adaptation of a calcifying tissue to mechanical perturbation is
directed by expression of osteopontin (opn), osteocalcin (oc) and bone
sialoprotein (bsp), and their interactions with mineral deposition. The
Specific Aims that will probe this structure-function relationship are to (1)
establish the expression pattern of the selected genes (opn, oc, bsp) as
compared to specific matrix proteins, matrix receptors, cytoskeletal proteins
and mineral formation in the three model systems; (2) determine the adaptive
response at the tissue level in terms of these parameters following
perturbation of the tendon model altered by a surgically-induced mechanical
change, and of the bone model through exercise; and (3) assess the adaptive
response at the organ level by determining the pattern of protein expression
and protein-mineral interactions in the phalanx-joint model placed under a
muscular load. This multifaceted approach will contribute to an increased
understanding of the chemistry, structure, organization, and interactions of
relevant molecules and cells that are critically important for the function of
mineralizing tissues and their adaptation under mechanical loading.
描述(由申请人提供):本提案的主要目标是
获取有关脊椎动物骨骼过程的新信息
分子和细胞水平的矿化作用。一种对
矿化意义重大,因为它是机械化的基础
身体的支撑,用于在机械约束下的骨骼适应,如
离子蓄水器,用于治疗、预防和治疗各种骨骼疾病
影响人类福祉的异常现象。拟议的研究将集中于
三个模型系统,来自正常火鸡的钙化肌腱,来自
训练小鼠,以及指骨和小关节的组织工程模型。
这些系统将在自然条件下和在发生变化后进行检查
改变作用在它们上面的机械力。结合了分子生物学,
将使用生化、免疫化学和结构技术来
确定细胞形成有机基质的过程
矿化,以及基质矿物的化学和结构性质
互动。需要检验的具体假设是:结构性和
钙化组织对机械扰动的功能适应是
骨桥蛋白(OPN)、骨钙素(Oc)和骨的表达
唾液蛋白(BSP)及其与矿物沉积的相互作用。这个
探讨这种结构-功能关系的具体目的是(1)
建立所选基因(opn、oc、bsp)的表达模式为
与特定的基质蛋白、基质受体、细胞骨架蛋白相比
以及三个模型体系中的成矿作用;(2)确定
根据以下这些参数在组织水平上的响应
由手术诱导的机械改变的肌腱模型的扰动
通过运动改变骨骼模型;以及(3)评估适应性
通过确定蛋白质表达模式在器官水平上的反应
和蛋白质-矿物相互作用的指骨-关节模型放置在一个
肌肉负荷。这种多方面的方法将有助于提高
对化学、结构、组织和相互作用的理解
对细胞的功能至关重要的相关分子和细胞
矿化组织及其在机械载荷下的适应性。
项目成果
期刊论文数量(37)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Analysis of human osteoarthritic connective tissue by laser capture microdissection and QRT-PCR.
通过激光捕获显微切割和 QRT-PCR 分析人类骨关节炎结缔组织。
- DOI:10.1080/03008200701692685
- 发表时间:2007
- 期刊:
- 影响因子:2.9
- 作者:Scharschmidt,Thomas;Jacquet,Robin;Laskovski,Jovan;Lowder,Elizabeth;Weiner,Scott;Landis,WilliamJ
- 通讯作者:Landis,WilliamJ
Tissue-engineered meniscal constructs.
组织工程半月板结构。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Schoenfeld,AndrewJ;Landis,WilliamJ;Kay,DavidB
- 通讯作者:Kay,DavidB
Quantitative determination of the mineral distribution in different collagen zones of calcifying tendon using high voltage electron microscopic tomography.
- DOI:
- 发表时间:1991
- 期刊:
- 影响因子:0
- 作者:B. McEwen;M. J. Song;W. Landis
- 通讯作者:B. McEwen;M. J. Song;W. Landis
Modified aminosilane substrates to evaluate osteoblast attachment, growth, and gene expression in vitro.
修饰氨基硅烷底物,用于评估体外成骨细胞附着、生长和基因表达。
- DOI:10.1002/jbm.a.30731
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Siperko,LM;Jacquet,R;Landis,WJ
- 通讯作者:Landis,WJ
Mineral characterization in calcifying tissues: atomic, molecular and macromolecular perspectives.
钙化组织中的矿物质特征:原子、分子和大分子视角。
- DOI:10.3109/03008209609005267
- 发表时间:1996
- 期刊:
- 影响因子:2.9
- 作者:Landis,WJ
- 通讯作者:Landis,WJ
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WILLIAM J LANDIS其他文献
WILLIAM J LANDIS的其他文献
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{{ truncateString('WILLIAM J LANDIS', 18)}}的其他基金
STUDY OF THE FORMATION OF ENAMEL CRYSTALLITES BY AMELOGENINS
釉蛋白形成牙釉质微晶的研究
- 批准号:
7598369 - 财政年份:2007
- 资助金额:
$ 30.83万 - 项目类别:
STUDY OF THE FORMATION OF ENAMEL CRYSTALLITES BY AMELOGENINS
釉蛋白形成牙釉质微晶的研究
- 批准号:
7357291 - 财政年份:2006
- 资助金额:
$ 30.83万 - 项目类别:
8th Conference - Chemistry & Biology Mineralized Tissue
第八届会议 - 化学
- 批准号:
6711598 - 财政年份:2004
- 资助金额:
$ 30.83万 - 项目类别:
Mineral-Matrix Relations in Calcifying Tissues
钙化组织中的矿物质-基质关系
- 批准号:
6625728 - 财政年份:1992
- 资助金额:
$ 30.83万 - 项目类别:
Mineral-Matrix Relations in Calcifying Tissues
钙化组织中的矿物质-基质关系
- 批准号:
6879176 - 财政年份:1992
- 资助金额:
$ 30.83万 - 项目类别:
MINERAL-MATRIX RELATIONS IN CALCIFYING TISSUES
钙化组织中矿物质与基质的关系
- 批准号:
2700217 - 财政年份:1992
- 资助金额:
$ 30.83万 - 项目类别:
MINERAL-MATRIX RELATIONS IN CALCIFYING TISSUES
钙化组织中矿物质与基质的关系
- 批准号:
2413975 - 财政年份:1992
- 资助金额:
$ 30.83万 - 项目类别:
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