Identifying the transcription master regulators in yeast

鉴定酵母中的转录主调控因子

• 批准号: 7157235
• 负责人: JIaqian Wu-Huber
• 金额: $ 4.6万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7157235
• 关键词: Saccharomyces cerevisiae; chromatin immunoprecipitation; computational biology; fungal genetics; fungal proteins; genetic regulation; microarray technology; molecular oncology; osmosis; postdoctoral investigator; transcription factor

DESCRIPTION (provided by applicant): Master regulators refer to transcription factors whose ectopic expression alone is capable of activating a cellular process. Master regulators have extremely important applications in regulating biological processes including cancer development and progression for therapeutic purposes. For instance, they can be used to bypass upstream deficiencies in a pathway and therefore restore the normal functions of the pathway. So far only a limited number of master regulators have been identified. In order to identify master regulators in other important eukaryotic processes, this research is to test the hypothesis that factors with the most incoming connections in a transcriptional network, namely target hubs, are likely master regulators. The transcription regulatory networks formed by ten key regulators of the osmotic response in Saccharomyces cerevisiae will be studied using chromatin immunoprecipitation and microarray analysis (chIP chip) with the goals of identifying target hubs. Whether these target hubs function as master regulators will be investigated. Finally, candidate target hubs and master regulators in additional cellular processes will be identified using the existing chIP chip data from other sources by computational strategies.

描述（由申请人提供）：主调节因子是指其异位表达单独能够激活细胞过程的转录因子。主调节器在调节生物过程（包括用于治疗目的的癌症发生和进展）方面具有极其重要的应用。例如，它们可用于绕过通路中的上游缺陷，从而恢复通路的正常功能。到目前为止，仅确定了有限数量的主监管机构。为了确定其他重要真核过程中的主调节因子，本研究旨在检验转录网络中具有最多传入连接的因子（即目标中枢）可能是主调节因子的假设。将使用染色质免疫沉淀和微阵列分析（chIP 芯片）来研究由酿酒酵母渗透反应的 10 个关键调节因子形成的转录调节网络，目的是确定目标中枢。这些目标枢纽是否充当主监管机构将受到调查。最后，将通过计算策略使用来自其他来源的现有芯片数据来识别其他细胞过程中的候选目标中枢和主调节器。