Identifying the transcription master regulators in yeast

描述(申请人提供)：主调控因子是指转录因子，其异位表达本身就能够激活细胞过程。MASTER调节剂在调节生物过程中具有极其重要的应用，包括癌症的发展和进展，用于治疗目的。例如，它们可以用来绕过通路的上游缺陷，从而恢复通路的正常功能。到目前为止，只确定了数量有限的主要监管者。为了确定在其他重要的真核过程中的主要调控因子，本研究验证了这样的假设，即在转录网络中具有最多传入连接的因子，即靶中心，可能是主调控因子。利用染色质免疫沉淀和微阵列分析(芯片)研究酿酒酵母渗透压反应的10个关键调控因子形成的转录调控网络，目的是识别目标枢纽。这些目标中心是否发挥了主要监管机构的作用，将得到调查。最后，通过计算策略，使用来自其他来源的现有芯片数据来识别额外蜂窝工艺中的候选目标中枢和主调节器。

DESCRIPTION (provided by applicant): Master regulators refer to transcription factors whose ectopic expression alone is capable of activating a cellular process. Master regulators have extremely important applications in regulating biological processes including cancer development and progression for therapeutic purposes. For instance, they can be used to bypass upstream deficiencies in a pathway and therefore restore the normal functions of the pathway. So far only a limited number of master regulators have been identified. In order to identify master regulators in other important eukaryotic processes, this research is to test the hypothesis that factors with the most incoming connections in a transcriptional network, namely target hubs, are likely master regulators. The transcription regulatory networks formed by ten key regulators of the osmotic response in Saccharomyces cerevisiae will be studied using chromatin immunoprecipitation and microarray analysis (chIP chip) with the goals of identifying target hubs. Whether these target hubs function as master regulators will be investigated. Finally, candidate target hubs and master regulators in additional cellular processes will be identified using the existing chIP chip data from other sources by computational strategies.