Identifying the transcription master regulators in yeast

描述（由申请人提供）：主调节因子是指其异位表达单独能够激活细胞过程的转录因子。主调节剂在调节生物过程中具有极其重要的应用，包括用于治疗目的的癌症发展和进展。例如，它们可以用于绕过途径中的上游缺陷，从而恢复途径的正常功能。到目前为止，只确定了数量有限的主调节器。为了确定其他重要的真核生物过程中的主调节因子，本研究旨在验证转录网络中具有最多传入连接的因子（即靶枢纽）可能是主调节因子的假设。将使用染色质免疫沉淀和微阵列分析（chIP芯片）研究酿酒酵母中渗透反应的10个关键调控因子形成的转录调控网络，其目标是识别靶枢纽。将调查这些目标枢纽是否作为主调节器。最后，将通过计算策略使用来自其他来源的现有chIP芯片数据来识别其他细胞过程中的候选目标枢纽和主调节器。

DESCRIPTION (provided by applicant): Master regulators refer to transcription factors whose ectopic expression alone is capable of activating a cellular process. Master regulators have extremely important applications in regulating biological processes including cancer development and progression for therapeutic purposes. For instance, they can be used to bypass upstream deficiencies in a pathway and therefore restore the normal functions of the pathway. So far only a limited number of master regulators have been identified. In order to identify master regulators in other important eukaryotic processes, this research is to test the hypothesis that factors with the most incoming connections in a transcriptional network, namely target hubs, are likely master regulators. The transcription regulatory networks formed by ten key regulators of the osmotic response in Saccharomyces cerevisiae will be studied using chromatin immunoprecipitation and microarray analysis (chIP chip) with the goals of identifying target hubs. Whether these target hubs function as master regulators will be investigated. Finally, candidate target hubs and master regulators in additional cellular processes will be identified using the existing chIP chip data from other sources by computational strategies.