Identification of Ebola Virus Entry Inhibitors

埃博拉病毒进入抑制剂的鉴定

• 批准号: 7155577
• 负责人: Arnab Basu
• 金额: $ 35.64万
• 依托单位: MICROBIOTIX, INC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7155577
• 关键词: cytotoxicity; infection; lead; library; life cycle; model; small molecule; virus infection mechanism

DESCRIPTION (provided by applicant): Ebola virus (EBOV) causes periodic outbreaks of severe viral hemorrhagic fevers in Africa with high mortality rates in infected patients. EBOV can be used in acts of bio-terrorism because of its highly infectious nature and stability in aerosolized form. It is classified as a Category A bioweapons agent by the Centers for Disease Control and Prevention (CDC). Currently, there is no FDA approved vaccine or antiviral drug that is effective against EBOV infections in humans. However, vaccines will only be partially effective in controlling an EBOV outbreak or bioterrorist attack since rapid progression of EBOV infection offers little opportunity for developing acquired immunity. Therefore, there is a critical need for to respond to postexposure or bioterrorist attack. EBOV infection is initiated by the fusion between viral and host cell membranes, which is mediated by the viral membrane glycoprotein (GP). This selective interaction between EBOV GP and host cell surface receptor molecules is essential for the initiation and establishment of the infection. Therefore, blocking of EBOV entry into its target cell will be advantageous since this will lead to suppression of viral infectivity early in its life cycle. Our objective is to discover and develop small molecule entry inhibitors against EBOV infection. Considering the aggressive nature of EBOV infection, and its extreme virulence, biosafety level 4 containment facilities are required to study the virus. To circumvent these stringent biohazard conditions, we will generate EBOV pseudotype virus as surrogate model to study EBOV attachment/entry. This model system allows the study of viral entry into the cell without replicating other aspects of the viral life cycle and therefore, can be performed in a biosafety level 2 facility. In Phase I, we will develop a high-throughput screening (HTS) assay utilizing the EBOV pseudotype virus to measure virus infection. This assay will be used to screen libraries of structurally diverse small molecules in "Microbiotix Inc's" repository, to identify potent inhibitors of EBOV entry. Inhibitors will be further confirmed for antiviral activity in a secondary EBOV pseudotype virus plaque assay, and infectious EBOV plaque assay. They will also be assessed for mammalian cytotoxicity. In Phase II, we will progress the most promising scaffolds through a rational drug design program, and lead compounds will be tested for efficacy and toxicity in animal models. The most active compound, with the least toxicity, will advance to IND enabling studies (Phase III). Therefore the specific aims of this Phase I application are to: 1. Develop a high-throughput screening (HTS) assay for selecting inhibitors of EBOV attachment/entry; 2. Screen a diverse compound library "Microbiotix Inc."s repository to identify and confirm selective inhibitors of EBOV; 3. EBOV inhibitor confirmation; and 4. Prioritize screening hits based on spectrum, cytotoxicity and mechanism. The end result of Phase 1 work will be identification of hits specific for EBOV entry inhibition.

描述（由申请人提供）：埃博拉病毒（EBOV）在非洲引起严重病毒性出血热的周期性爆发，感染患者死亡率很高。埃博拉病毒因其高度传染性和雾化形式的稳定性而可用于生物恐怖主义行为。它被美国疾病控制与预防中心 (CDC) 归类为 A 类生物武器制剂。目前，尚无 FDA 批准的疫苗或抗病毒药物可有效对抗人类埃博拉病毒感染。然而，疫苗在控制埃博拉病毒爆发或生物恐怖袭击方面只能部分有效，因为埃博拉病毒感染的快速发展几乎没有机会形成获得性免疫。因此，迫切需要对暴露后或生物恐怖袭击做出反应。埃博拉病毒感染是由病毒与宿主细胞膜之间的融合引发的，该融合由病毒膜糖蛋白（GP）介导。 EBOV GP 和宿主细胞表面受体分子之间的这种选择性相互作用对于感染的启动和建立至关重要。因此，阻断 EBOV 进入其靶细胞将是有利的，因为这将导致在其生命周期早期抑制病毒感染性。我们的目标是发现和开发针对埃博拉病毒感染的小分子进入抑制剂。考虑到埃博拉病毒感染的侵袭性及其极高的毒力，需要生物安全4级遏制设施来研究该病毒。为了规避这些严格的生物危害条件，我们将生成 EBOV 假型病毒作为替代模型来研究 EBOV 附着/进入。该模型系统允许研究病毒进入细胞而不复制病毒生命周期的其他方面，因此可以在生物安全 2 级设施中进行。在第一阶段，我们将开发一种利用 EBOV 假型病毒来测量病毒感染的高通量筛选 (HTS) 检测方法。该测定将用于筛选“Microbiotix Inc”存储库中结构多样的小分子文库，以鉴定埃博拉病毒进入的有效抑制剂。抑制剂的抗病毒活性将在次级 EBOV 假型病毒空斑测定和传染性 EBOV 空斑测定中得到进一步证实。还将评估它们的哺乳动物细胞毒性。在第二阶段，我们将通过合理的药物设计方案开发最有前途的支架，并在动物模型中测试先导化合物的功效和毒性。最活跃且毒性最小的化合物将进入 IND 启用研究（第三阶段）。因此，第一阶段申请的具体目标是： 1. 开发一种高通量筛选（HTS）测定法，用于选择 EBOV 附着/进入的抑制剂； 2. 筛选多样化的化合物库“Microbiotix Inc.”的存储库，以鉴定和确认EBOV的选择性抑制剂； 3. EBOV抑制剂确认； 4. 根据谱、细胞毒性和机制优先筛选命中。第一阶段工作的最终结果将是识别针对 EBOV 进入抑制的特异性命中。