MOLECULAR EPIDEMIOLOGY OF STLV-I IN WILD AND CAPTIVE SOOTY MANGABEYS

野生和圈养乌白眉猴 STLV-I 的分子流行病学

• 批准号: 7349040
• 负责人: VICKI L TRAINA-DORGE
• 金额: $ 6.54万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349040
• 关键词: MOLECULAR; EPIDEMIOLOGY; STLV; WILD; CAPTIVE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A study was conducted to evaluate the prevalence and diversity of simian T cell lymphotropic virus (STLV) within the long established Tulane National Primate Research Center (TNPRC) colony of sooty mangabeys (SM; Cercocebus atys). Serological analysis determined that 22 of 39 animals (56%) were positive for STLV-1. A second group of thirteen SM bush-meat samples from Sierra Leone, Africa was also included and tested only by PCR. Twenty-two of 39 captive animals (56%) and 3 of 13 bush-meat samples (23%) were positive for STLV-1 by PCR. Nucleotide sequencing and phylogenetic analysis of viral strains obtained demonstrated that STLV-Ism strains from the TNPRC colony and Sierra Leone formed a single cluster together with the previously reported STLV-1sm strain from Yerkes National Primate Research Center (YNPRC).These data confirm an African origin for TNPRC STLV-1sm and suggest a Sierra Leone origin for the SM colonies in the United States. The TNPRC STLV-1sm strains further divided into two subclusters suggesting STLV-1sm infection of two original founder SMs at the time of their importation into the United States. STLV-1sm diversity in the TNPRC colony matches the already reported colony's high diversity of SIVsm. The lack of correlation between SIVsm lineage and STLV-1sm subcluster distribution of TNPRC strains suggest that intracolony transmission of both viruses were independent events.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。进行了一项研究，以评估在杜兰国家灵长类动物研究中心（TNPRC）长期建立的白眉猴（SM; Cercocebus atys）群体中猴T细胞嗜淋巴细胞病毒（STLV）的流行率和多样性。血清学分析确定39只动物中有22只（56%）为STLV-1阳性。来自非洲塞拉利昂的第二组13个SM肉样品也包括在内，仅通过PCR进行检测。22 39圈养动物（56%）和3 13个猪肉样品（23%）的STLV-1的PCR阳性。核苷酸序列分析和系统发育分析表明，STLV-Ism株从TNPRC殖民地和塞拉利昂形成一个单一的集群一起与以前报道的STLV-1 sm株从耶基斯国家灵长类动物研究中心（YNPRC）。这些数据证实了非洲起源的TNPRC STLV-1 sm和建议塞拉利昂起源的SM殖民地在美国。TNPRC STLV-1 sm毒株进一步分为两个亚群，表明两种原始创始SM在输入美国时感染了STLV-1 sm。STLV-1 sm多样性在TNPRC殖民地匹配已经报道的殖民地的SIVsm的高多样性。TNPRC株的SIVsm谱系与STLV-1 sm亚群分布之间缺乏相关性，表明两种病毒的集落内传播是独立的事件。