SIMIAN VARICELLA VIRUS INFECTION AND LATENCY IN THE NONHUMAN PRIMATE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 猴水痘病毒（SVV）感染灵长类动物与人水痘带状疱疹病毒（VZV）感染具有相同的临床、病理、免疫学和病毒学特征。 通过将SVV血清阴性猴暴露于SVV感染的笼友猴，可在食蟹猴或非洲绿色猴（AGM）中建立潜伏性SVV感染。 今年，我们进行了三项AGM实验的一部分。两组潜伏感染猴水痘病毒（SVV），并利用两种不同的治疗方法进行可能的再激活治疗。动物GV .仅用单次剂量（200 cGy）的X射线照射，动物HC用单次剂量（200 cGy）的X射线照射，然后用他克莫司（80-300 mg/kg/天）处理。第三组动物分组饲养，每组中有一只动物急性感染SVV。这一组通过了急性期，正在发展潜伏期。在三个月内，这最后一组将接受如上所述的照射，并每天给予免疫抑制药物泼尼松（1 mg/kg/天）治疗，作为病毒再活化的可能触发因素。正在对采集的样本进行病毒载量、组织病理学变化和SVV再激活证据分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Simian varicella virus (SVV) infection of primates shares clinical, pathological, immunological and virological features of varicella zoster virus (VZV) infection of humans. Latent SVV infection can be established in cynomologous or African green monkeys (AGM) by exposing SVV-seronegative monkeys to an SVV infected cage-mate monkey. This year we conducted portions of three experiments with AGMs. Two groups were latently infected with simian varicella virus (SVV) and treated for possible reactivation utilizing two different treatments. Animals GV . were only irradiated with a single dose (200cGy of X-rays, animals HC were were irradiated with a single dose (200 cGy) of X-irradiation and then treated with tacrolimus (80-300 mg/kg/day). The third group of animals were group housed and one animal from every group acutely infected with SVV. This group passed the acute phase and is in the process of developing latency. In three months, this final group will undergo irradiation, as described above, and be given daily treatment with the immunosuppressive drug, prednisone (1 mg/kg/day), as a possible trigger of virus reactivation. Samples taken are being analyzed for virus load, histopathological changes and evidence of SVV reactivation.