SIMIAN VARICELLA VIRUS INFECTION AND LATENCY IN THE NONHUMAN PRIMATE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 灵长类动物感染水痘病毒(SVV)具有人类水痘带状疱疹病毒(VZV)感染的临床、病理、免疫学和病毒学特征。通过将SVV血清阴性的猴子暴露于感染SVV的笼配猴，可在细胞学或非洲绿猴(AGM)中建立潜伏的SVV感染。今年，我们用股东大会进行了三个实验的一部分。两组被潜伏感染猴水痘病毒(SVV)，并使用两种不同的治疗方法进行可能的再激活治疗。动物GV。只接受单次剂量(200cGyX射线)照射，动物HC接受单次剂量(200cGyX射线)照射，然后给予他克莫司(80~300 mg/kg/d)治疗。第三组动物被分组饲养，每组中有一只动物急性感染SVV。这组人度过了急性期，正在形成潜伏期。在三个月内，这最后一组人将接受如上所述的放射治疗，并每天接受免疫抑制药物泼尼松(1 mg/kg/天)的治疗，以可能触发病毒的重新激活。正在对采集的样本进行病毒载量、组织病理学变化和SVV重新激活的证据进行分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Simian varicella virus (SVV) infection of primates shares clinical, pathological, immunological and virological features of varicella zoster virus (VZV) infection of humans. Latent SVV infection can be established in cynomologous or African green monkeys (AGM) by exposing SVV-seronegative monkeys to an SVV infected cage-mate monkey. This year we conducted portions of three experiments with AGMs. Two groups were latently infected with simian varicella virus (SVV) and treated for possible reactivation utilizing two different treatments. Animals GV . were only irradiated with a single dose (200cGy of X-rays, animals HC were were irradiated with a single dose (200 cGy) of X-irradiation and then treated with tacrolimus (80-300 mg/kg/day). The third group of animals were group housed and one animal from every group acutely infected with SVV. This group passed the acute phase and is in the process of developing latency. In three months, this final group will undergo irradiation, as described above, and be given daily treatment with the immunosuppressive drug, prednisone (1 mg/kg/day), as a possible trigger of virus reactivation. Samples taken are being analyzed for virus load, histopathological changes and evidence of SVV reactivation.