SIMIAN VARICELLA VIRUS INFECTION AND LATENCY IN THE NONHUMAN PRIMATE

非人类灵长类动物中的猿水痘病毒感染和潜伏期

• 批准号: 7958580
• 负责人: VICKI L TRAINA-DORGE
• 金额: $ 5.8万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958580
• 关键词: Acute; Animals; Cercopithecus pygerythrus; Chickenpox; Clinical; Computer Retrieval of Information on Scientific Projects Database; Dose; Funding; Grant; Herpesvirus Type 3; Housing; Human; Immunosuppressive Agents; Institution; Monkeys; Partner in relationship; Pharmaceutical Preparations; Phase; Prednisone; Primates; Process; Research; Research Personnel; Resources; Roentgen Rays; Sampling; Source; Tacrolimus; United States National Institutes of Health; Viral load measurement; Virus; Virus Diseases; irradiation; nonhuman primate; research study; simian virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Simian varicella virus (SVV) infection of primates shares clinical, pathological, immunological and virological features of varicella zoster virus (VZV) infection of humans. Latent SVV infection can be established in cynomologous or African green monkeys (AGM) by exposing SVV-seronegative monkeys to an SVV infected cage-mate monkey. This year we conducted portions of three experiments with AGMs. Two groups were latently infected with simian varicella virus (SVV) and treated for possible reactivation utilizing two different treatments. Animals GV . were only irradiated with a single dose (200cGy of X-rays, animals HC were were irradiated with a single dose (200 cGy) of X-irradiation and then treated with tacrolimus (80-300 mg/kg/day). The third group of animals were group housed and one animal from every group acutely infected with SVV. This group passed the acute phase and is in the process of developing latency. In three months, this final group will undergo irradiation, as described above, and be given daily treatment with the immunosuppressive drug, prednisone (1 mg/kg/day), as a possible trigger of virus reactivation. Samples taken are being analyzed for virus load, histopathological changes and evidence of SVV reactivation.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 灵长类动物的猿水痘病毒（SVV）感染与人类水痘带状疱疹病毒（VZV）感染具有相同的临床、病理、免疫学和病毒学特征。 通过将 SVV 血清阴性猴暴露于 SVV 感染的笼伴猴，可以在食蟹猴或非洲绿猴 (AGM) 中建立潜伏 SVV 感染。 今年，我们用 AGM 进行了三个实验的部分内容。两组均潜伏感染猿水痘病毒（SVV），并使用两种不同的治疗方法进行可能的重新激活治疗。动物 GV 。仅用单剂量（200cGy X 射线）照射，HC 动物用单剂量（200 cGy）X 射线照射，然后用他克莫司（80-300 mg/kg/天）治疗。第三组动物分群饲养，每组中一只动物急性感染 SVV。该组已过了急性期，正处于潜伏期。三个月后，这最后一组动物会 如上所述，接受放射治疗，并每天接受免疫抑制药物泼尼松（1 毫克/公斤/天）治疗，作为病毒重新激活的可能触发因素。正在分析采集的样本的病毒载量、组织病理学变化和 SVV 重新激活的证据。