STLV-1 GENETIC DIVERSITY IN CAYO SANTIAGO RHESUS MACAQUES

圣地亚哥岛恒河猴的 STLV-1 遗传多样性

• 批准号: 7349113
• 负责人: VICKI L TRAINA-DORGE
• 金额: $ 3.1万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349113
• 关键词: STLV; GENETIC; DIVERSITY; CAYO; SANTIAGO

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Naturally occurring simian T cell lymphotropic (STLV-I) virus strains have been identified and isolated from Old World, nonhuman primates and include: African green monkeys, rhesus macaques, sooty mangabey monkeys, and baboons. A unique free ranging rhesus macaque colony at Cayo Santiago, Puerto Rico, established in 1938 and a part of the Carribean Primate Research Center was shown serologically, by Dr. Sariol Curbelo, to have a high percentage of the animals that were infected with STLV-1. We began molecular studies to evaluate the diversity of STLVs within this population of animals. Blood samples from serologically positive animals were sent to TNPRC for isolation of peripheral blood mononuclear cells (PBMC) and their cellular DNA. These DNAs were amplified by PCR for both STLV-I envelope (env) and LTR, nucleotide sequencing conducted, and phylogenetic comparisons with known HTLV-I and STLV-I isolates made. The comparisons of the first few STLV-I virus isolates for env sequences, clustered together and near to other Macaca mulatta isolates, but distinct from other Old World primate families. We are continuing and extending these studies with further characterization of nucleotide sequences of each isolate and making phylogenetic comparisons.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。天然存在的猿猴 T 细胞嗜淋巴细胞 (STLV-I) 病毒株已从东半球非人类灵长类动物中鉴定和分离，包括：非洲绿猴、恒河猴、乌白眉猴和狒狒。 Sariol Curbelo 博士的血清学结果表明，波多黎各圣地亚哥岛于 1938 年建立的一个独特的自由放养恒河猴群落是加勒比灵长类动物研究中心的一部分，该群落中感染 STLV-1 的动物比例很高。我们开始分子研究来评估该动物群体中 STLV 的多样性。血清学阳性动物的血液样本被送往 TNPRC，用于分离外周血单核细胞 (PBMC) 及其细胞 DNA。通过 STLV-I 包膜 (env) 和 LTR 的 PCR 扩增这些 DNA，进行核苷酸测序，并与已知的 HTLV-I 和 STLV-I 分离株进行系统发育比较。前几个 STLV-I 病毒分离株的 env 序列比较，它们聚集在一起并靠近其他猕猴分离株，但与其他旧大陆灵长类家族不同。我们正在继续并扩展这些研究，进一步表征每个分离株的核苷酸序列并进行系统发育比较。