STLV-1 GENETIC DIVERSITY IN CAYO SANTIAGO RHESUS MACAQUES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。已从东半球非人类灵长类动物中鉴定和分离出自然发生的猿猴T细胞淋巴(STLV-I)病毒株，包括：非洲绿猴、恒河猴、煤烟山猴和狒狒。波多黎各卡约圣地亚哥的一个独特的自由放养猕猴群体成立于1938年，是加勒比海灵长类动物研究中心的一部分，萨里奥尔·柯贝洛博士通过血清学证明，感染STLV-1的动物比例很高。我们开始了分子研究，以评估这一动物种群中STLV的多样性。血清阳性动物的血液样本被送往TNPRC进行外周血单个核细胞(PBMC)及其细胞DNA的分离。用聚合酶链式反应扩增出STLV-I包膜(Env)和LTR的DNA，进行核苷酸序列测定，并与已知的HTLV-I和STLV-I分离株进行系统发育比较。最初几个STLV-I病毒分离株的env序列的比较，聚在一起并接近其他猕猴分离株，但不同于其他东半球灵长类动物家族。我们正在继续和扩展这些研究，进一步描述每个分离物的核苷酸序列，并进行系统发育比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Naturally occurring simian T cell lymphotropic (STLV-I) virus strains have been identified and isolated from Old World, nonhuman primates and include: African green monkeys, rhesus macaques, sooty mangabey monkeys, and baboons. A unique free ranging rhesus macaque colony at Cayo Santiago, Puerto Rico, established in 1938 and a part of the Carribean Primate Research Center was shown serologically, by Dr. Sariol Curbelo, to have a high percentage of the animals that were infected with STLV-1. We began molecular studies to evaluate the diversity of STLVs within this population of animals. Blood samples from serologically positive animals were sent to TNPRC for isolation of peripheral blood mononuclear cells (PBMC) and their cellular DNA. These DNAs were amplified by PCR for both STLV-I envelope (env) and LTR, nucleotide sequencing conducted, and phylogenetic comparisons with known HTLV-I and STLV-I isolates made. The comparisons of the first few STLV-I virus isolates for env sequences, clustered together and near to other Macaca mulatta isolates, but distinct from other Old World primate families. We are continuing and extending these studies with further characterization of nucleotide sequences of each isolate and making phylogenetic comparisons.