Next Generation Nano-prism based LSPR chemical sensors.

下一代基于纳米棱镜的 LSPR 化学传感器。

• 批准号: 7161313
• 负责人: JEFFREY N ANKER
• 金额: $ 4.88万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-05 至 2008-12-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7161313
• 关键词: Aging; Alzheimer' s Disease; Amyloid; Binding; Binding Proteins; Biological Markers; Cerebrospinal Fluid; Cerebrum; Chemicals; Chemistry; Coupling; Deposition; Devices; Diagnosis; Disease; Electromagnetic Fields; Equilibrium; Evaluation; Goals; In Situ; Ionic Strengths; Kinetics; Lasers; Legal patent; Ligands; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Molecular; Monitor; Pharmaceutical Preparations; Proteins; Relative (related person); Research; Shapes; Signal Transduction; Specificity; Stretching; Structure; Surface; Techniques; Temperature; Testing; Thick; Time; base; improved; instrument; ionization; nano; next generation; sensor; size

DESCRIPTION (provided by applicant): This project will develop the next generation of LSPR sensors based on plasmon resonance of metallic nanoprism arrays. LSPR sensors, invented and developed in the Van Duyne lab, enable high sensitivity measurement of molecular concentration and in situ characterization of binding constants. Recently LSPR sensors measured the concentration of "amyloid derived diffusable ligands" (ADDLs), a promising biomarker for and possible cause of Alzheimer's disesase, in the cerebral spinal fluid of Alzheimer's and aging controls; preliminary results showed higher levels in Alzheimer's patents and further tests are in progress. This project enhances current techniques by 1) Optimizing nanoprism shape and chemistry, and modulating binding by changing pH, temperature, and ionic strength. 2) Depositing nanoprisms onto flexible and stretchable surfaces to modulate nanoprism spacing and electromagnetic field coupling. 3) Interfacing the LSPR substrates with mass spectroscopy (MS) instruments for complimentary binding studies and MS identification. These sensors hold promise for study of ADDL chemistry, as well as for Alzheimer's diagnosis and continuing evaluation of treatment. The techniques may also be used for other biomarkers and diseases.

描述(申请人提供)：该项目将开发基于金属纳米棱镜阵列的等离子体激元共振的下一代LSPR传感器。由Van Duyne实验室发明和开发的LSPR传感器能够实现高灵敏度的分子浓度测量和结合常数的现场表征。最近，LSPR传感器测量了阿尔茨海默病和老年对照组脑脊液中“淀粉样衍化可扩散配体”(ADDLS)的浓度；初步结果显示，阿尔茨海默病专利中的水平更高，进一步的测试正在进行中。该项目通过1)优化纳米棱镜的形状和化学，并通过改变pH、温度和离子强度来调节结合，从而增强了现有的技术。2)在柔性和可伸展的表面沉积纳米棱镜，以调节纳米棱镜间距和电磁场耦合。3)将LSPR底物与质谱仪(MS)连接，以进行免费的结合研究和MS鉴定。这些传感器有望用于Addl化学研究，以及阿尔茨海默氏症的诊断和持续的治疗评估。该技术也可用于其他生物标志物和疾病。