Next Generation Nano-prism based LSPR chemical sensors.

下一代基于纳米棱镜的 LSPR 化学传感器。

• 批准号: 7323310
• 负责人: JEFFREY N ANKER
• 金额: $ 3.32万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-05 至 2008-07-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7323310
• 关键词: Aging; Alzheimer' s Disease; Amyloid; Binding; Binding Proteins; Biological Markers; Cerebrospinal Fluid; Cerebrum; Chemicals; Chemistry; Coupling; Deposition; Devices; Diagnosis; Disease; Electromagnetic Fields; Equilibrium; Evaluation; Goals; In Situ; Ionic Strengths; Kinetics; Lasers; Legal patent; Ligands; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Molecular; Monitor; Pharmaceutical Preparations; Proteins; Relative (related person); Research; Shapes; Signal Transduction; Specificity; Stretching; Structure; Surface; Techniques; Temperature; Testing; Thick; Time; base; improved; instrument; ionization; nano; next generation; sensor; size

This project will develop the next generation of LSPR sensors based on plasmon resonance of metallic nanoprism arrays. LSPR sensors, invented and developed in the Van Duyne lab, enable high sensitivity measurement of molecular concentration and in situ characterization of binding constants. Recently LSPR sensors measured the concentration of "amyloid derived diffusable ligands" (ADDLs), a promising biomarker for and possible cause of Alzheimer's disesase, in the cerebral spinal fluid of Alzheimer's and aging controls; preliminary results showed higher levels in Alzheimer's patents and further tests are in progress. This project enhances current techniques by 1) Optimizing nanoprism shape and chemistry, and modulating binding by changing pH, temperature, and ionic strength. 2) Depositing nanoprisms onto flexible and stretchable surfaces to modulate nanoprism spacing and electromagnetic field coupling. 3) Interfacing the LSPR substrates with mass spectroscopy (MS) instruments for complimentary binding studies and MS identification. These sensors hold promise for study of ADDL chemistry, as well as for Alzheimer's diagnosis and continuing evaluation of treatment. The techniques may also be used for other biomarkers and diseases.

该项目将开发基于金属等离子体共振的下一代LSPR传感器。 纳米棱镜阵列。在货车Duyne实验室发明和开发的LSPR传感器实现了高灵敏度 测量分子浓度和原位表征结合常数。最近LSPR 传感器测量“淀粉样蛋白衍生的可扩散配体”（ADDLs）的浓度，ADDLs是一种有前途的生物标志物， 在阿尔茨海默氏症患者和老年对照组的脑脊液中发现阿尔茨海默氏症的可能病因; 初步结果显示，老年痴呆症患者中的水平较高，进一步的测试正在进行中。这个项目 1）优化纳米棱镜形状和化学，并通过以下方式调节结合： 改变pH、温度和离子强度。2)将纳米棱镜沉积在柔性和可拉伸的 表面以调制纳米棱镜间距和电磁场耦合。3)连接LSPR 用于互补结合研究和MS的质谱（MS）仪器 识别.这些传感器有望用于ADDL化学研究以及阿尔茨海默氏症的诊断 并继续评估治疗。该技术也可用于其他生物标志物和疾病。

项目成果

Gas sensing with high-resolution localized surface plasmon resonance spectroscopy.

• DOI: 10.1021/ja1074272
• 发表时间: 2010-12-15
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Bingham JM;Anker JN;Kreno LE;Van Duyne RP
• 通讯作者: Van Duyne RP

Noninvasively Imaging pH at the Surface of Implanted Orthopedic Devices with X-ray Excited Luminescence Chemical Imaging.

• DOI: 10.1021/acssensors.9b00962
• 发表时间: 2019-09-27
• 期刊: ACS SENSORS
• 影响因子: 8.9
• 作者: Uzair, Unaiza;Benza, Donald;Behrend, Caleb J.;Anker, Jeffrey N.
• 通讯作者: Anker, Jeffrey N.

A conformation- and ion-sensitive plasmonic biosensor.

• DOI: 10.1021/nl103994w
• 发表时间: 2011-03-09
• 期刊: Nano letters
• 影响因子: 10.8
• 作者: Hall WP;Modica J;Anker J;Lin Y;Mrksich M;Van Duyne RP
• 通讯作者: Van Duyne RP