Next Generation Nano-prism based LSPR chemical sensors.

该项目将开发基于金属等离子体共振的下一代LSPR传感器 纳米棱镜阵列。由Van Duyne实验室发明和开发的LSPR传感器可以实现高灵敏度 分子浓度的测量和结合常数的原位表征。最近的LSPR 传感器测量了“淀粉样蛋白衍生的可扩散配体”(ADDLS)的浓度，这是一种很有前途的生物标志物 阿尔茨海默病和老年对照组脑脊液中阿尔茨海默病的病因和可能的病因； 初步结果显示，阿尔茨海默病的专利水平更高，进一步的测试正在进行中。这个项目 通过1)优化纳米棱镜的形状和化学并通过以下方式调节结合来增强当前的技术 改变pH值、温度和离子强度。2)在柔性和可伸缩的表面沉积纳米管 用于调制纳米棱镜间距和电磁场耦合的表面。3)对接LSPR 带有质谱仪的底物，用于免费的结合研究和MS 身份证明。这些传感器有望用于其他化学研究以及阿尔茨海默氏症的诊断 以及持续的治疗评估。该技术也可用于其他生物标志物和疾病。

This project will develop the next generation of LSPR sensors based on plasmon resonance of metallic nanoprism arrays. LSPR sensors, invented and developed in the Van Duyne lab, enable high sensitivity measurement of molecular concentration and in situ characterization of binding constants. Recently LSPR sensors measured the concentration of "amyloid derived diffusable ligands" (ADDLs), a promising biomarker for and possible cause of Alzheimer's disesase, in the cerebral spinal fluid of Alzheimer's and aging controls; preliminary results showed higher levels in Alzheimer's patents and further tests are in progress. This project enhances current techniques by 1) Optimizing nanoprism shape and chemistry, and modulating binding by changing pH, temperature, and ionic strength. 2) Depositing nanoprisms onto flexible and stretchable surfaces to modulate nanoprism spacing and electromagnetic field coupling. 3) Interfacing the LSPR substrates with mass spectroscopy (MS) instruments for complimentary binding studies and MS identification. These sensors hold promise for study of ADDL chemistry, as well as for Alzheimer's diagnosis and continuing evaluation of treatment. The techniques may also be used for other biomarkers and diseases.