Next Generation Nano-prism based LSPR chemical sensors.

项目描述（申请人提供）：本项目将开发基于金属纳米棱镜阵列等离子体共振的下一代LSPR传感器。LSPR传感器由Van Duyne实验室发明和开发，能够高灵敏度测量分子浓度和原位表征结合常数。最近，LSPR传感器测量了阿尔茨海默病患者和衰老对照组脑脊液中“淀粉样蛋白衍生的可扩散配体”（ADDLs）的浓度，这是一种有希望的阿尔茨海默病生物标志物和可能的阿尔茨海默病病因；初步结果显示阿尔茨海默氏症的专利含量较高，进一步的测试正在进行中。该项目通过1)优化纳米棱镜的形状和化学性质，以及通过改变pH值、温度和离子强度来调节结合来改进现有技术。2)在柔性和可拉伸表面上沉积纳米棱镜，以调节纳米棱镜间距和电磁场耦合。3)用质谱（MS）仪器连接LSPR底物，进行互补结合研究和质谱鉴定。这些传感器有望用于ADDL化学研究，以及阿尔茨海默病的诊断和治疗的持续评估。该技术也可用于其他生物标志物和疾病。

DESCRIPTION (provided by applicant): This project will develop the next generation of LSPR sensors based on plasmon resonance of metallic nanoprism arrays. LSPR sensors, invented and developed in the Van Duyne lab, enable high sensitivity measurement of molecular concentration and in situ characterization of binding constants. Recently LSPR sensors measured the concentration of "amyloid derived diffusable ligands" (ADDLs), a promising biomarker for and possible cause of Alzheimer's disesase, in the cerebral spinal fluid of Alzheimer's and aging controls; preliminary results showed higher levels in Alzheimer's patents and further tests are in progress. This project enhances current techniques by 1) Optimizing nanoprism shape and chemistry, and modulating binding by changing pH, temperature, and ionic strength. 2) Depositing nanoprisms onto flexible and stretchable surfaces to modulate nanoprism spacing and electromagnetic field coupling. 3) Interfacing the LSPR substrates with mass spectroscopy (MS) instruments for complimentary binding studies and MS identification. These sensors hold promise for study of ADDL chemistry, as well as for Alzheimer's diagnosis and continuing evaluation of treatment. The techniques may also be used for other biomarkers and diseases.