Cell Identity and differentiation in stimulus response

刺激反应中的细胞识别和分化

• 批准号: 7133001
• 负责人: JOSE R DINNENY
• 金额: $ 4.6万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7133001
• 关键词: Aftercare; Behavior; Cell Separation; Cells; Complex; Data; Depth; Development; Exposure to; Future; Genes; Genetic; Goals; Image; Imagery; Individual; Lead; Methodology; Monitor; Mouse-ear Cress; Organ; Output; Perception; Phenotype; Physiology; Plant Model; Plant Roots; Plants; Play; Quality Control; Regulation; Research Personnel; Response to stimulus physiology; Role; Signal Transduction; Stimulus; System; Work; base; cell type; cost; design; mutant; new technology; research study; response; tool

Organs are composed of cells with distinct identities designed to interpret stimuli in specialized ways and coordinately fulfill complex functions. In order to understand how cells perform these specialized functions I plan to study the mechanisms by which cell identity alters the quality and quantity of a stimulus response. I propose to use Fluorescent Activated Cell Sorting (FACS) in combination with cell-type specific fluorescent marker lines to isolate individual cell types of the root after treatment with a stimulus. Using microarrays, I will then analyze the transcriptional changes that occur in each cell type of the root and identify genes that demonstrate cell-type specific transcriptional responses. These data will not only lead to a better understanding of the relationship between cell identity and stimulus perception and response but will also lead to the development of markers to monitor this regulation and marker-based mutant screens to identify the genetic mechanisms that control it. In addition, I propose to collaborate in the development of a high-throughput platform for visualizing root development and physiology termed the "root array" that will facilitate the work proposed here and contribute to the design of future experiments.

器官是由具有不同身份的细胞组成的，这些细胞被设计成以特定的方式来解释刺激