Cellular Fluorescence-Contractility Imaging System

细胞荧光收缩成像系统

• 批准号: 6877423
• 负责人: MICHAEL E MENDELSOHN
• 金额: $ 10.75万
• 依托单位: TUFTS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877423
• 关键词: bioimaging /biomedical imaging; biological signal transduction; biomedical equipment purchase; calcium; cardiac myocytes; fluorescence; heart contraction; muscle relaxation; protein localization; protein structure function; vascular smooth muscle

DESCRIPTION (provided by applicant): The Molecular Cardiology Research Institute (MCRI) at the New England Medical Center is devoted to the exploration of the molecular mechanisms that underlie human cardiovascular diseases. MCRI investigators use in vitro molecular biological, cell biological and transgenic approaches to identify and validate new therapeutic targets. The investigators within the MCRI pursue varied, yet complementary avenues of research--the vast majority of which are NIH-supported. Two broad areas of research within the MCRI include vascular and cardiomyocyte biology. We examine the normal and pathophysiologic regulation and function of vasomotor tone, cardiomyocyte hypertrophy and apoptosis, cardiac development and cell proliferation. A common focus for many of the NIH-funded MCRI Investigators--those who comprise the Investigators in this proposal--is mechanisms that regulate contraction and relaxation of cardiac and vascular myocytes. In order to understand the molecular events that regulate cardiac myocyte and vascular smooth muscle cell contraction and relaxation, cellular and biochemical events are studied extensively in cultured cardiac and vascular cells, which are routinely generated, passaged and stored in our cell culture facilities. This is an efficient system for the study of cardiovascular signaling that minimizes the use of intact animals. However, at present we do not have the capacity to provide sophisticated physiological analysis of cell signaling and cell contraction and relaxation in single cells. The MCRI has thus identified the need for an imaging apparatus that can simultaneously measure cell contraction and relaxation and perform highresolution fluorescence imaging in real-time. These goals are focused on acquiring new technology in two areas: calcium imaging related to cell contraction, and signal transduction pathways and fluorescence detection of proteins to study their activity and intracellular localization. Thus, based on ongoing programs and increasing needs within the MCRI for the capacity to study cardiac and vascular cell physiology, the purpose of this proposal is to gain the support needed to build a highly integrated cell imaging system to measure real-time single cell contraction relaxation and simultaneous calcium transients. Acquisition of this system will facilitate the extensive and complementary studies of cardiovascular signal transduction within our institution.

描述（由申请人提供）：新英格兰医学中心的分子心脏病学研究所（MCRI）致力于探索人类心血管疾病的分子机制。 MCRI研究人员使用体外分子生物学，细胞生物学和转基因方法来识别和验证新的治疗靶标。 MCRI追求的研究人员各种各样的研究途径各不相同，但大多数是NIH支持的。 MCRI中的两个广泛研究领域包括血管和心肌细胞生物学。我们检查了血管舒缩张力，心肌细胞肥大和凋亡，心脏发育和细胞增殖的正常和病理生理调节和功能。对于许多由NIH资助的MCRI研究者来说，这是该提案中研究人员的一个普遍重点是调节心脏和血管心肌细胞收缩和放松的机制。为了了解调节心肌细胞和血管平滑肌细胞收缩和放松的分子事件，在培养的心脏和血管细胞中进行了广泛的研究，细胞和生化事件经常在我们的细胞培养设施中产生，被传染和储存。这是研究心血管信号传导的有效系统，可最大程度地减少完整动物的使用。但是，目前，我们没有能力对单个细胞的细胞信号传导和细胞收缩以及松弛的复杂生理分析。因此，MCRI已经确定了需要同时测量细胞收缩和放松并实时执行高分辨率荧光成像的成像设备的需求。这些目标的重点是在两个领域获取新技术：与细胞收缩有关的钙成像，信号转导途径和蛋白质的荧光检测以研究其活性和细胞内定位。 因此，基于MCRI内正在进行的计划和研究心脏和血管细胞生理能力的需求的增加，该建议的目的是获得建立高度集成的细胞成像系统所需的支持，以衡量实时单细胞收缩放松和同时钙瞬时。获取该系统将有助于我们机构内心血管信号转导的广泛和互补研究。