Using lattice light sheet microscopy to determine how the Spindle Assembly Checkpoint turns off

使用点阵光片显微镜确定主轴装配检查点如何关闭

• 批准号: 2869034
• 金额: --
• 依托单位: Institute of Cancer Research
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2869034
• 关键词: Using; lattice; light; sheet; microscopy

Unattached kinetochores prevent anaphase by generating an inhibitor composed of the MAD2, BUB3 and BUBR1 Spindle Assembly Checkpoint proteins. Kinetochores that stably attach to microtubules no longer generate the inhibitor but we do not understand how microtubule attachment does this. Is one attached microtubule sufficient, or are several required? Is the ability of microtubules to 'strip' kinetochores of the inhibitor proteins fast enough? To answer these questions we need to image microtubule attachment to kinetochores in real-time and quantify the changes in MAD2 and BUBR1 at kinetochores. We can now do this using lattice-lightsheet microscopy and endogenously tagged proteins.

未连接的动粒通过产生由MAD 2、BUB 3和BUBR 1纺锤体组装检查点蛋白组成的抑制剂来防止后期。稳定附着在微管上的动粒不再产生抑制剂，但我们不知道微管附着是如何做到这一点的。一个微管就足够了，还是需要几个？微管“剥离”抑制蛋白的动粒的能力足够快吗？为了回答这些问题，我们需要实时成像微管附着在着丝粒上，并量化着丝粒上MAD 2和BUBR 1的变化。我们现在可以使用格子光片显微镜和内源性标记蛋白质来做到这一点。