Advanced lattice light sheet microscope optimised for biological imaging

针对生物成像优化的先进点阵光片显微镜

• 批准号: LE220100138
• 负责人: Prof Graham Lieschke
• 金额: $ 53.34万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2022
• 资助国家: 澳大利亚
• 起止时间: 2022-05-06 至 2022-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE220100138
• 关键词: Advanced; lattice; light; sheet; microscope

This project provides a lattice light sheet microscope optimised for biological applications and user utility. Lattice light sheet microscopy combines optical, physical and technical attributes that achieve high spatio-temporal resolution imaging of biological specimens for long periods with minimal phototoxicity, making practical for the first time high-resolution longitudinal volumetric analyses of living specimens. It is suitable for imaging cultured cells, organoids, small embryos, and small model organisms such as zebrafish larvae at cellular and subcellular resolution. Expected outcomes are high impact discoveries, training opportunities, international collaborations and publications addressing fundamental questions in biology.

该项目提供了一个晶格光片显微镜优化的生物应用和用户实用程序。点阵光片显微镜结合了光学，物理和技术属性，实现了生物标本的高时空分辨率成像长时间与最小的光毒性，使实用的第一次高分辨率纵向体积分析活标本。它适用于以细胞和亚细胞分辨率对培养细胞、类器官、小胚胎和小模型生物（如斑马鱼幼虫）进行成像。预期成果是高影响力的发现，培训机会，国际合作和解决生物学基本问题的出版物。