LC/quadrupole ion trap mass spectroscopy system

LC/四极杆离子阱质谱系统

• 批准号: 6880314
• 负责人: ROBERT M STRAUBINGER
• 金额: $ 31.87万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6880314
• 关键词: biomedical equipment purchase; mass spectrometry; pharmacology; proteomics

DESCRIPTION (provided by applicant): For the selection of nine core NIH-supported projects described in this proposal, the liquid chromatography/quadrupole ion trap (LC-ion trap MS-n) mass spectroscopy system proposed would provide major enhancement. For the 14 co-investigators, key features of the proposed instrument include high combined sensitivity/scan speed in full-scan mode, multi-stage fragmentation (Ms-n), and data-dependent acquisition in automated scan mode. The projects have in common the need for identification of pharmacological agents and their in vivo metabolites, as well as their effect on cellular responses at the protein level. A major use of the instrument will be the identification and characterization of low copy number proteins and peptides in biological matrices, using methods we are developing. The specific instrument we propose is the Thermo Finnigan LCQ Deca XP ion trap MS. It would be unique in the extended geographic region of Western New York, and would be the only instrument of its kind available on a shared basis for investigators who require the direct access to the instrument and the extended use time necessary to develop new analytical approaches for protein and peptide analysis. It will be integrated into the existing Pharmaceutical Sciences mass spectrometry facility, consisting of two triple quadrupole LC/MS-MS instruments. This facility provides instrument access to a diverse population of researchers engaged in basic, applied, and clinical biomedical research, as well as analytical mass spectrometry. The facility is highly utilized, providing investigators with 4,000 hours of data acquisition on the existing LC/triple quad instruments in the last calendar year. Mass spectrometric analysis is a mainstay of many NIH-supported projects, but this region and university lacks the complementary capabilities that the Deca XP ion trap instrument will provide. Proteomics and protein chemical analysis projects are hindered by the lack of an instrument well suited for identification and characterization of proteins and peptides. Furthermore, no facility exists to provide the extended access necessary to support the development of new methods for quantitation of low-copy cellular protein transcripts or therapeutically important protein drugs. The proposed instrumentation would also enable more facile identification and characterization of small-molecule drugs and their metabolites. Given the involvement of our investigators in studies of high clinical relevance, the proposed instrument would have direct, major impact on the development of new therapies for life-threatening diseases.

描述（由申请人提供）：对于本提案中描述的九个NIH支持的核心项目的选择，所提出的液相色谱/四极离子阱（LC-离子阱MS-n）质谱系统将提供重大增强。对于14名共同研究者，拟议仪器的关键特征包括全扫描模式下的高组合灵敏度/扫描速度、多级碎片（Ms-n）和自动扫描模式下的数据依赖性采集。这些项目的共同点是需要鉴定药物及其体内代谢物，以及它们在蛋白质水平上对细胞反应的影响。该仪器的一个主要用途将是使用我们正在开发的方法鉴定和表征生物基质中的低拷贝数蛋白质和肽。我们提出的具体仪器是Thermo Finnigan LCQ Deca XP离子阱MS。它在西纽约的扩展地理区域中是独一无二的，并且将是唯一的同类仪器，可用于需要直接访问仪器和延长使用时间以开发蛋白质和肽分析新分析方法的研究人员。它将被集成到现有的药物科学质谱设施，包括两个三重四极杆LC/MS-MS仪器。该设施为从事基础，应用和临床生物医学研究以及分析质谱的各种研究人员提供仪器访问。该设施的利用率很高，在过去一个日历年里，为调查人员提供了4 000小时的现有LC/三重四仪器数据采集。质谱分析是许多NIH支持的项目的支柱，但该地区和大学缺乏Deca XP离子阱仪器将提供的补充能力。蛋白质组学和蛋白质化学分析项目由于缺乏非常适合于蛋白质和肽的鉴定和表征的仪器而受到阻碍。此外，没有设施存在提供必要的扩展访问，以支持开发新的方法来定量低拷贝细胞蛋白质转录本或治疗上重要的蛋白质药物。拟议的仪器还将使小分子药物及其代谢物的鉴定和表征更加容易。鉴于我们的研究人员参与了具有高度临床相关性的研究，拟议的仪器将对危及生命的疾病的新疗法的开发产生直接的重大影响。

项目成果

Proteomic expression profiling of Haemophilus influenzae grown in pooled human sputum from adults with chronic obstructive pulmonary disease reveal antioxidant and stress responses.

• DOI: 10.1186/1471-2180-10-162
• 发表时间: 2010-06-01
• 期刊: BMC microbiology
• 影响因子: 4.2
• 作者: Qu J;Lesse AJ;Brauer AL;Cao J;Gill SR;Murphy TF
• 通讯作者: Murphy TF

A rapid, reproducible, on-the-fly orthogonal array optimization method for targeted protein quantification by LC/MS and its application for accurate and sensitive quantification of carbonyl reductases in human liver.

• DOI: 10.1021/ac902314m
• 发表时间: 2010-04-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Cao, Jin;Covarrubias, Vanessa M.;Straubinger, Robert M.;Wang, Hao;Duan, Xiaotao;Yu, Haoying;Qu, Jun;Blanco, Javier G.
• 通讯作者: Blanco, Javier G.

Determination of the Proteomic Response to Lapatinib Treatment using a comprehensive and reproducible ion-current-based proteomics strategy.

使用全面且可重复的基于离子流的蛋白质组学策略确定对拉帕替尼治疗的蛋白质组学反应。

• DOI: 10.14302/issn.2326-0793.jpgr-13-257
• 发表时间: 2013
• 期刊: Journal of proteomics and genomics research
• 作者: O'Connell,Kathleen;Li,Jun;Engler,Frank;Hennessy,Kim;O'Neill,Fiona;Straubinger,RobertM;Qu,Jun;O'Connor,Robert
• 通讯作者: O'Connor,Robert