CORE--TRANSGENIC ANIMAL MODEL DEVELOPMENT

具体目标 1.继续开发新的转基因动物模型，用于研究衰老生物学机制。 2.提供一个资源，使华盛顿大学健康科学教师参与各种老龄化项目，利用基因工程技术回答有关衰老生物学的基本问题。 3.在构建靶向载体，可注射DNA构建体和诱导型启动子系统方面提供资源和专业知识，用于生成衰老生物学的遗传改变动物模型。 4.加速和加强冷冻保存胚胎和精子从有用的基因改变小鼠模型的老化储存库存储和保护住房资源。 5.为转基因和胚胎干细胞技术的研究员和初级教员提供培训资源。

SPECIFIC AIMS 1. Continue to develop new genetically altered animal models for studying mechanisms of the biology of aging. 2. Provide a resource that enables University of Washington Health Sciences faculty involved in various aging projects to utilize genetic engineering technology for answering basic questions about the biology of aging. 3. Provide a resource and expertise in the construction of targeting vectors, injectable DNA constructs and inducible promoters systems for generating genetically altered animal models of the biology of aging. 4. Accelerate and enhance cryopreservation of embryos and sperm from useful genetically altered mouse models of aging for repository storage and conservation of housing resources. 5. Provide a training resource for fellows and junior faculty in transgenic and embryonic stem cell technology.