DEVELOPMENT OF A FLUORESCENCE BASED TESTSYSTEM FOR GPCR ACTIVATION

开发基于荧光的 GPCR 激活测试系统

• 批准号: 7181390
• 负责人: MARTIN J LOHSE
• 金额: $ 1.3万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181390
• 关键词: bioimaging /biomedical imaging; biomedical resource; image processing; technology /technique development; transmission electron microscopy

DESCRIPTION: The National Center for Microscopy and Imaging Research (NCMIR) was established to develop computer aided advanced microscopy for acqusition of structural and functional data in the dimensional range of 1 nm3 to 100 um3. With novel specimen staining methods, imaging instruments and computational capabilities, researchers are addressing the next great biological challenges in the post-genomic age by situating proteins and macromolecular complexes in their cellular and tissue environements. Resource instruments include intermediate high voltage transmission electron microscopes (IVEMs) and high-speed large-format laser-scanning light microscopes specially modified for "meso-scale" biological microscopy. Our core technological research is directed towards developing technologies that will contribute to structural neurobiology, cell and molecular biology. With NCMIR's participation in the Biomedical Informatics Research Network (BIRN), we are scaling up our ability to image at high resolution across wide expanses of brain tissue to reveal structural and molecular in mouse models of human disease. Our collaboration, service, training and dissemination programs expand the use of these technologies to maximize their value to the biomedical community. Core biological projects include development of new staining methods for IVEM and for correlated light and IVEM analyses of specimens to examine the architecture of neuronal systems and the dynamics of subcellular processes. Core research is tightly linked to collaborative projects, in areas such as animal of neurodegenerative diseases, stroke, heart disease, cancer, infectious diseases and diabetes. The capabilites and research accomplishments anticipated in specimen preparation are augmented by continued development of instruments and image processing facilities. We emphasize the use of computer-aided methods for enhancing image contrast and reconstructing larger structural complexes using 3D tomographic and serial thick section analysis. Extraction of 3D information on a large scale will be accelerated through the development of high-throughput methods for tomographic reconstruction, analysis and visualization. We continue to provide researchers greater access to the Resource through development of web-based remote control systems for the IVEM's and interfaces for computational grids and distributed databases via high-speed networks. We will expand outreach efforts via web-based tutorials, software and microscopic data repositories linked to other biological databases.

产品说明：国家显微镜和成像研究中心（NCMIR）的成立是为了开发计算机辅助先进显微镜，以获取1 nm 3至100 um 3尺寸范围内的结构和功能数据。随着新的标本染色方法，成像仪器和计算能力，研究人员正在解决下一个巨大的生物学挑战，在后基因组时代，通过定位蛋白质和大分子复合物在他们的细胞和组织结构。资源仪器包括中高压透射电子显微镜（IVEMS）和高速大幅面激光扫描光学显微镜，专门为“中尺度”生物显微镜而修改。我们的核心技术研究旨在开发有助于结构神经生物学，细胞和分子生物学的技术。随着NCMIR参与生物医学信息学研究网络（BIRN），我们正在扩大我们在广泛的脑组织中以高分辨率成像的能力，以揭示小鼠的结构和分子 人类疾病的模型。我们的合作，服务，培训和传播计划扩大了这些技术的使用，以最大限度地发挥其对生物医学界的价值。核心生物学项目包括开发IVEM和相关光的新染色方法 和标本的IVEM分析，以检查神经元系统的架构和亚细胞过程的动力学。核心研究与动物神经退行性疾病、中风、心脏病、癌症、传染病和糖尿病等领域的合作项目密切相关。通过仪器和图像处理设备的不断发展，标本制备的能力和研究成果得到了增强。我们强调使用计算机辅助的方法来增强图像对比度和重建较大的结构复杂的三维断层扫描和连续厚切片分析。通过开发用于层析重建、分析和可视化的高通量方法，将加速大规模提取3D信息。我们继续为研究人员提供更多的资源，通过开发基于网络的远程控制系统的IVEM的和接口的计算网格和分布式数据库通过高速网络。我们将通过基于网络的教程、软件和与其他生物学数据库链接的微观数据库扩大外联工作。