INTRACELLULAR MODIFICATIONS OF HUMAN APOLIPOPROTEIN E

人载脂蛋白 E 的细胞内修饰

• 批准号: 7182222
• 负责人: VASSILIS I ZANNIS
• 金额: $ 0.69万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7182222
• 关键词: biomedical equipment resource; biomedical resource; mass spectrometry; method development

DESCRIPTION: MALDI mass spectrometry has become one of the two important methods driving the application of mass spectrometry in biochemistry. A problem that stands in the way of broad application is the extensive metastable-ion decay exhibited by some molecules. This is a particular problem when a class of molecules has one or more labile groups that undergo reactions with significantly lower fragmentation barriers than those for analytically useful fragmentations. An example is the loss of water, for which there is little information content. The problem is made worse when a mixture is encountered. The composite fragmentation is difficult, if not impossible, to assign. A promising development to overcome this limitation is to initiate the desorption process in the presence of a bath gas. This idea will be pursued for the analysis of samples containing oligosaccharides, glyco and phosphopeptides, sialylated glycolipids, sulfated oligosaccharides, and peptides containing oxidized cysteine residues. Many of these samples are of interest to collaborators of the BU research resource.

描述：MALDI质谱已成为推动质谱在生物化学中应用的两种重要方法之一。 阻碍广泛应用的一个问题是某些分子表现出广泛的亚稳态离子衰变。 当一类分子具有一个或多个不稳定基团时，与分析上有用的碎片相比，这些基团的碎片势垒显着降低，这是一个特殊的问题。 一个例子是水的流失，对此的信息内容很少。 当遇到混合物时，问题会变得更糟。 复合碎片即使不是不可能，也很难分配。 克服这一限制的一个有希望的发展是在浴气体存在下启动解吸过程。 这一想法将用于分析含有寡糖、糖肽和磷酸肽、唾液酸化糖脂、硫酸化寡糖以及含有氧化半胱氨酸残基的肽的样品。 波士顿大学研究资源的合作者对其中许多样本感兴趣。