TIME RESOLVED SAXS OF MICROSECOND PROTEIN FOLDING INTERMEDIATES

微秒蛋白质折叠中间体的时间分辨萨克斯

• 批准号: 7182104
• 负责人: Osman Bilsel
• 金额: $ 3.1万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7182104
• 关键词: biomedical resource; biophysics; clinical research

DESCRIPTION (provided by applicant): We propose to continue operation of the Biophysics Collaborative Access Team (BioCAT) undulator beamline 18-1D at the Advanced Photon Source, Argonne National Laboratory. It's mission is to provide a national research resource for the study of partially ordered biological molecules, complexes of biomolecules and cellular structures under conditions similar to those present in living cells. The goal of such research is to understand the detailed mechanisms of action of such systems at the molecular level. The techniques employed are non-crystalline x-ray diffraction, x-ray scattering and x-ray absorption emission spectroscopy. The BioCAT facilities provide a resource unique on the continent; only SPring-8 in Japan and the ESRF in France provide similar capabilities. New thrust areas include molecular shape determination from small angle scattering, high-resolution spectroscopy, micro-emission/absorption spectroscopy, and micro-diffraction. Major enhancements are planned for the areas of fiber diffraction, especially for the muscle diffraction program. Research and development efforts are also proposed in several areas directly related to improving data quality, reliability and efficiency of operation. Novel modes of operation are proposed that improve throughput and the quality of training.

描述（由申请人提供）：我们建议在阿贡国家实验室的高级光子源继续运行生物物理学合作访问小组（BioCAT）波荡器光束线18-1D。它的使命是为研究部分有序的生物分子、生物分子复合物和在类似于活细胞中存在的条件下的细胞结构提供国家研究资源。这种研究的目标是在分子水平上了解这些系统的详细作用机制。所采用的技术是非晶X-射线衍射，X-射线散射和X-射线吸收发射光谱。BioCAT设施提供了非洲大陆独一无二的资源;只有日本的SPring-8和法国的ESRF提供类似的能力。新的推力领域包括小角散射，高分辨率光谱，微发射/吸收光谱和微衍射的分子形状测定。计划对纤维衍射领域，特别是肌肉衍射程序进行重大改进。还建议在与提高数据质量、可靠性和业务效率直接相关的若干领域开展研究和开发工作。提出了新的操作模式，以提高吞吐量和训练质量。