Haemophilus parainfluenzae and respiratory disease

副流感嗜血杆菌与呼吸道疾病

• 批准号: 7072405
• 负责人: WADE A NICHOLS
• 金额: $ 21.45万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7072405
• 关键词: bacteria; biofilm; glycolipids; human; influenza; respiratory epithelium; tissues

DESCRIPTION (provided by applicant): The objective of this research is to study the pathogenic ability of the bacterium Haemophilus parainfluenzae. H. parainfluenzae is frequently isolated from the sites of respiratory disease in humans, however, little direct evidence exists that demonstrate that the bacterium causes damage to the host tissue. This study proposes an investigation of the ability of H. parainfluenzae strains to bind to and invade human respiratory epithelium and to cause inflammation by the epithelial tissue. Evidence that H. parainfluenzae can adhere to epithelium and elicit an inflammatory response would support the hypothesis that the bacterium is an opportunistic pathogen of compromised respiratory tissue. Additional studies will be performed to establish profiles about the lipooligosaccharide, outer membrane proteins, and fimbriae of all strains studied. This information sheds light on the degree of surface variation in the species and also may help to predict molecules involved in adherence to human cells. Finally, the ability of H. parainfluenzae to form biofilms will be determined. Production of biofilms has been shown to be beneficial for pathogens of the human respiratory tract. By completing these studies, we hope to gain a better understanding of the basic molecular biology of the bacterium H. parainfluenzae and as well as determining if the bacterium possesses the abilities.

描述（由申请方提供）：本研究的目的是研究副流感嗜血杆菌的致病能力。H.副流感病毒经常从人的呼吸道疾病部位分离出来，然而，几乎没有直接证据表明该细菌对宿主组织造成损害。本研究对H.副流感病毒株结合并侵入人呼吸道上皮，并引起上皮组织的炎症。证据表明，H。副流感病毒可粘附于上皮并引发炎性反应，这将支持该细菌是受损呼吸组织的机会致病菌的假设。将进行额外的研究，以建立关于所有研究菌株的脂寡糖、外膜蛋白和菌毛的特征。这些信息揭示了物种表面变异的程度，也可能有助于预测参与粘附人类细胞的分子。最后，对H.将确定副流感病毒形成生物膜的能力。生物膜的产生已被证明对人类呼吸道的病原体是有益的。通过完成这些研究，我们希望能够更好地了解细菌H的基本分子生物学。并确定该细菌是否具有这种能力。