Analytical and Phenotyping Core

核心C：分析和表型分析核心 分析核心的目的是提供交感神经系统的最先进的生化分析。 功能，包括测量儿茶酚胺、其代谢物和去甲肾上腺素清除率， 溢出这一资源对我们的新的 涉及自主神经系统的疾病，如多巴胺-β-羟化酶缺乏症。一 在本核心中已经建立了各种技术来支持各个项目，包括 测定血浆容量和动态液体转移。所有四个项目都将利用这一资源。 交感神经活动的生化评价将补充自主神经评价的其他方法 在临床核心中详细描述。此外，我们还实施了动物表型鉴定设施， 用于评估人类自主神经功能的方法。这包括，除其他外， 监测清醒的非限制性小鼠的动脉内血压，监测肾交感神经 活动和高保真心电图，在完整的和sinoartic-denervated小鼠。转基因小鼠 在翻译自主知识方面越来越重要，这个表型小鼠实验室将是 在这方面至关重要。

Core C: Analytical and Phenotyping Core The purpose of the Analytical Core is to provide state-of -the art biochemical analysis of sympathetic function, including measurements of catecholamines, their metabolites, and norepinephrine clearance and spillover. This resource has been critical to the recognition and characterization by our group of new disorders involving the autonomic nervous system, such ads dopamine-beta-hydroxylase deficiency. A variety of techniques have been established in this Core to support individual projects, including the determination of plasma volume and dynamic fluid shifts. All four projects will utilize this resource. Biochemical evaluation of sympathetic activity will complement other methods of autonomic evaluation detailed in the Clinical Core. In addition, we have implemented an animal phenotyping facility, adapting the methods developed to evaluate autonomic function in humans, to the mice. This includes, among others, monitoring intraarterial blood pressure in awake unrestricted mice, monitoring renal sympathetic nerve activity and high fidelity ECG, in intact and sinoartic-denervated mice. Genetically-modified mice are increasingly important in translating autonomic knowledge, and this phenotyping mice laboratory will be essential in this regard.