ZEISS META 510 CONFOCAL IMAGING SYSTEM: CVD HEART

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：生物化学和分子生物学系是共聚焦激光扫描显微镜共享仪器设施的所在地，目前配备蔡司LSM 410。这台仪器已有近10年的历史，被医学院、埃普利癌症及相关疾病研究所、UNMC/埃普利癌症中心和奥马哈退伍军人管理医院的研究人员广泛使用。该设施位于达勒姆研究中心，这是一个新的10层基础研究设施。该中心包含136个实验室模块，外加一层专用于核心设施，包括共聚焦显微镜核心。实验室模块的使用将仅限于当前和新招募的研究人员，他们有积极的研究资助（NIH或同等），平均每年20万美元/模块。我们的目标是继续提供最先进的设施，以提高UNMC的研究质量，并扩大我们核心的能力，以容纳越来越多的用户。这将通过购买全电动共聚焦LSM系统来实现，该系统具有最新的分析软件、激光器和附件，可提供最大的分辨率和荧光发射分离，以及获取每个像素光谱特征的探测器。这将允许UNMC的用户组执行优化的共定位，并方便地获得有关蛋白质-蛋白质相互作用和活细胞中蛋白质运输的信息。本建议中列出的项目是从目前总共19个主要用户中的12个主要用户中选出的。预计在未来3-5年内，随着UNMC校园的研究工作通过新招聘和偶尔用户干部的持续增长而扩大，用户池将翻一番。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Department of Biochemistry and Molecular Biology is the home of a shared instrument facility for confocal laser scanning microscopy and is currently equipped with Zeiss LSM 410. This instrument, now almost 10 years old, is used extensively by researchers from the College of Medicine, the Eppley Institute for Research in Cancer and Related Diseases, the UNMC/Eppley Cancer Center and the Veterans' Administration Hospital in Omaha. The facility is located in the Durham Research Center, a new 10-story basic research facility. This Center contains 136 laboratory modules plus one floor dedicated to core facilities including the Confocal Micorscopy Core. Occupancy of lab modules will be limited to current and newly recruited investigators with active research grants (NIH or equivalent) averaging ~$200,000/year/module. Our objective is to continue providing state-of-the-art facilities to enhance the quality of research at UNMC and to expand the capacity of our core to accommodate an increasing pool of users. This will be done through the purchase of a fully motorized confocal LSM system with up-to-date analytic software, lasers and accessories that give maximum resolution and separation of fluorescent emissions and detectors that acquire spectral signatures for each pixel. This will allow the user group at UNMC to perform optimized co- localization, and to conveniently obtain information about protein-protein interactions and protein trafficking in live cells. The projects listed in this proposal are selected from those of the 12 major users out of a current total of 19. It is anticipated that the user pool will double over the next 3-5 years with the expansion of the research effort on the UNMC campus through new recruitment and from the continued growth of a cadre of occasional users.