LSM 710 Zeiss Confocal Microscope

LSM 710 蔡司共焦显微镜

• 批准号: 7795000
• 负责人: JUDITH K CHRISTMAN
• 金额: $ 46.11万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2011-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795000
• 关键词: Arts; Cells; Confocal Microscopy; Core Facility; Detection; Disease; Equipment; Floor; Funding; Goals; Grant; Housing; Laboratories; Lasers; Life; Malignant Neoplasms; Medicine; Microscope; Minor; Recruitment Activity; Research; Research Institute; Research Personnel; Research Project Grants; Scientist; Services; UNMC Eppley Cancer Center; United States National Institutes of Health; college; fluorophore; instrument; meetings; programs; protein protein interaction; protein transport

DESCRIPTION (provided by applicant): The Confocal Microscopy Core Facility (CMCF) is housed in the Durham Research Center (DRC), which opened in 2006. The CMCF was initiated at UNMC in 1996 and has served the research goals of >80 scientists since then. It is used extensively by scientists from the College of Medicine, the Eppley Institute for Research in Cancer and Related Diseases and the UNMC/ Eppley Cancer Center. The CMCF now maintains two confocal microscopes, a Zeiss LSM 410 and a Zeiss LSM 510 Meta. In 2006, the SIG program funded the purchase of an LSM 510 which was upgraded to an LSM 510Ma with funds provided by Vice Chancellor for Research at UNMC. The LSM 510M was put into service in early 2007, shortly after the CMCF moved to the DRC. This 10 story building, containing 136 laboratories will now become DRC I; an adjacent tower (DRC II) containing an additional 98 laboratories with added core facility space on the first floor will open in May. With few exceptions, occupancy of the lab modules is limited to current and newly recruited investigators with active research grants (NIH or equivalent) averaging > $200,000/year/module. This means that the CMCF can realistically expect an increase in the number of NIH funded investigators with need to access the CMCF for their NIH funded research. In 2006, we planned to keep both the LSM 410 and 510M in use to cover the needs of an increased number of investigators. However, only a few of our users are willing to use the limited capacity of this instrument. In contrast, the LSM 510M is in almost constant use. A technician was added to the staff so that we now have two knowledgeable people to provide assistance to investigators on weekdays from 7:30 AM to 7:30 PM (or later in special situations). Before grant dead-lines and meetings, there is often a 4-5 day wait to access the LSM 510M. Thus, it is imperative to increase our capacity. Our objective with this request for an LSM 710 is to continue providing state of the art facilities and to expand the capacity of our core to accommodate an increasing pool of NIH users. Confocal usage (hrs) in the CMCF has almost doubled since 2005 and continues to grow, making the purchase of the LSM 710 a critical need. The requested LSM 710 with Quasar spectral detection, 34 spectral detection channels and 6 laser lines- 405, 458, 488, 514, 561 and 633 nm and incubation equipment will allow the users to perform optimized co- localization of multiple fluorophores and to conveniently obtain information about protein-protein interactions and protein trafficking in live cells. The projects detailed in this proposal are selected from those of 11 major users and 23 minor users.

描述（由申请人提供）：共聚焦显微镜核心设施（CMCF）位于2006年开放的达勒姆研究中心（DRC）。CMCF于1996年在UNMC启动，自那时以来已为超过80名科学家的研究目标服务。它被来自医学院，Eppley癌症和相关疾病研究所和UNMC/ Eppley癌症中心的科学家广泛使用。CMCF现在拥有两台共聚焦显微镜，一台Zeiss LSM 410和一台Zeiss LSM 510 Meta。2006年，SIG计划资助购买LSM 510，并由UNMC副校长提供资金升级为LSM 510 Ma。LSM 510 M于2007年初投入使用，当时CMCF刚刚搬到刚果民主共和国。这座包含136个实验室的10层建筑现在将成为DRC I;相邻的塔楼（DRC II）包含额外的98个实验室，一楼增加了核心设施空间，将于5月开放。除了少数例外，实验室模块的占用仅限于当前和新招募的研究人员，平均每年/模块的有效研究赠款（NIH或同等机构）> 200，000美元。这意味着CMCF可以切实地预期NIH资助的研究人员数量的增加，他们需要访问CMCF进行NIH资助的研究。2006年，我们计划继续使用LSM 410和510 M，以满足越来越多调查人员的需要。然而，只有少数用户愿意使用该仪器的有限容量。相比之下，LSM 510 M几乎一直在使用。工作人员中增加了一名技术人员，因此我们现在有两名知识渊博的人员在工作日上午7：30至下午7：30（或在特殊情况下更晚）为研究者提供协助。在批准截止日期和会议之前，通常需要等待4-5天才能访问LSM 510 M。因此，必须提高我们的能力。我们申请LSM 710的目的是继续提供最先进的设施，并扩大我们的核心容量，以适应越来越多的NIH用户。自2005年以来，CMCF的共焦使用率（小时数）几乎翻了一番，并且还在继续增长，这使得购买LSM 710成为一个关键需求。所要求的LSM 710具有Quasar光谱检测、34个光谱检测通道和6条激光线（405、458、488、514、561和633 nm）以及孵育设备，将允许用户执行多个荧光团的优化共定位，并方便地获得有关活细胞中蛋白质-蛋白质相互作用和蛋白质运输的信息。本建议书中详述的项目是从11个主要用户和23个次要用户的项目中选出的。