ZEISS META 510 CONFOCAL IMAGING SYSTEM: PROSTATE, PANCREATIC CANCER, LYMPHOMA

蔡司 META 510 共焦成像系统：前列腺癌、胰腺癌、淋巴瘤

• 批准号: 7334972
• 负责人: JUDITH K CHRISTMAN
• 金额: $ 14.79万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7334972
• 关键词: biochemistry; biomedical facility; confocal scanning microscopy; lasers; lymphoma; medicine; molecular biology; neoplasm /cancer; pancreas neoplasms; prostate; protein protein interaction; university; veterans

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Department of Biochemistry and Molecular Biology is the home of a shared instrument facility for confocal laser scanning microscopy and is currently equipped with Zeiss LSM 410. This instrument, now almost 10 years old, is used extensively by researchers from the College of Medicine, the Eppley Institute for Research in Cancer and Related Diseases, the UNMC/Eppley Cancer Center and the Veterans' Administration Hospital in Omaha. The facility is located in the Durham Research Center, a new 10-story basic research facility. This Center contains 136 laboratory modules plus one floor dedicated to core facilities including the Confocal Micorscopy Core. Occupancy of lab modules will be limited to current and newly recruited investigators with active research grants (NIH or equivalent) averaging ~$200,000/year/module. Our objective is to continue providing state-of-the-art facilities to enhance the quality of research at UNMC and to expand the capacity of our core to accommodate an increasing pool of users. This will be done through the purchase of a fully motorized confocal LSM system with up-to-date analytic software, lasers and accessories that give maximum resolution and separation of fluorescent emissions and detectors that acquire spectral signatures for each pixel. This will allow the user group at UNMC to perform optimized co- localization, and to conveniently obtain information about protein-protein interactions and protein trafficking in live cells. The projects listed in this proposal are selected from those of the 12 major users out of a current total of 19. It is anticipated that the user pool will double over the next 3-5 years with the expansion of the research effort on the UNMC campus through new recruitment and from the continued growth of a cadre of occasional users.

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：生物化学和分子生物学系是共焦激光扫描显微镜的共用仪器设施，目前配备了蔡司LSM 410。这种仪器现在已经有近10年的历史，被来自医学院、艾普利癌症及相关疾病研究所、UNMC/艾普利癌症中心和奥马哈退伍军人管理医院的研究人员广泛使用。该设施位于达勒姆研究中心，这是一个新的10层基础研究设施。该中心包括136个实验室单元，外加一层专门用于包括共焦显微镜核心在内的核心设施。实验室模块的占用将仅限于现有和新招聘的研究人员，他们拥有活跃的研究补助金(NIH或同等资助金)，平均为200,000美元/年/模块。我们的目标是继续提供最先进的设施，以提高军事和军事中心的研究质量，并扩大我们的核心能力，以容纳越来越多的用户。这将通过购买配备最新分析软件的全电动共焦激光测距系统、提供最大分辨率和荧光发射分离的激光器和配件以及获取每个像素光谱特征的探测器来实现。这将使UNMC的用户组能够进行优化的共定位，并方便地获得关于活细胞中蛋白质-蛋白质相互作用和蛋白质运输的信息。本提案所列的项目是从12个主要用户中挑选出来的，目前共有19个用户。预计未来3-5年内，随着通过新的招聘扩大军事管理委员会园区的研究工作，以及临时用户队伍的持续增长，用户群将翻一番。