BD FACSCANTO FLOW CYTOMETER: AIDS

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：拉霍亚分子医学研究所(LJIMM)是一家非营利性研究机构，从事干细胞生物学、哮喘/过敏、趋化因子生物学、传染病疫苗和疗法、癌症和哮喘的免疫疗法、信号转导、血管生物学和血管生成等领域的基础和应用研究。流式细胞仪是LJIMM所有研究人员在研究项目中使用的常见设备之一。该研究所目前有一台FACScan，其能力仅限于检测最多三种颜色，不能满足调查人员目前的需求。一些研究人员在他们的工作中使用校外多色流式细胞仪。然而，在校外地点使用设备并不总是可行的，因为成本高、实验的时间敏感性以及各研究所之间运送样品的困难。因此，FACScan的核心主要用户群体确定了教员的具体需求，并从BD Bioscitions公司选择了合适的流式细胞仪，即FACSCanto，作为目前和未来需求的理想系统。在这笔赠款中，我们申请资金购买带有FACSDiva计划的FACSCanto系统和两个工作站，总成本为192,172美元。通过购买FACSCanto，我们将建立一个流式细胞仪核心设施，为核心建立一个内部咨询委员会(IAC)，并制定设备使用政策。LJIMM致力于开发核心设施，并提供必要的财务支持(年度服务合同和兼职研究助理的工资)和基础设施(服务器、局域网和计算机)要求。与我们现有的显微镜和体内成像核心相结合，拟议的流式细胞仪将是一个重要的补充，以最大限度地发挥我们现有研究计划的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The La Jolla Institute for Molecular Medicine (LJIMM) is a nonprofit research institute conducting basic and applied research in the fields of stem cell biology, asthma/allergy, chemokine biology, vaccines and therapeutics for infectious diseases, immuno therapies for cancer and asthma, signal transduction, vascular biology and angiogenesis. The flow cytometer is one of the common pieces of equipment that all the investigators use for their research projects at LJIMM. The institute currently has a FACScan with capabilities limited to detecting a maximum of three colors and is inadequate for the present needs of the investigators. Several investigators utilize off-campus multi- color flow cytometers for their work. However, it is not always practical to use equipment at off-campus locations due to high costs, time sensitive nature of the experiments, and the difficulty in transportation of samples between the institutes. Therefore, a core group of primary users of FACScan determined the specific needs of the faculty and have chosen an appropriate flow cytometer, namely FACSCanto, from BD Biosciences as the ideal system for present and future requirements. In this grant, we are requesting funds to purchase a FACSCanto system with FACSDiva program and two work stations at a total cost of $192,172. With the purchase of a FACSCanto, we will establish a Flow Cytometry Core facility, an Internal Advisory Committee (IAC) for the core and set policies for the use of the equipment. LJIMM is committed to developing core facilities and providing the necessary financial support (annual service contract and salary for a part-time research associate) and infrastructural (Server, LAN and computers) requirements. In combination with our existing Microscopy and Intravital Imaging Core, the proposed flow cytometer will be an important addition to maximize the impact of our existing research programs.