X-SPECT FOR FUNCTIONAL & ANATOMIC IMAGING IN VIVO: PHARMACOLOGY

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：本共享仪器资助申请书要求使用伽玛医疗设备公司制造的X-SPECT成像系统。X-SPECT(Tm)是一种结合了X射线计算机断层扫描(CT)和单光子发射计算机断层扫描(SPECT)的双模式小动物成像系统。因此，X-SPECT(Tm)能够生成麻醉下完整动物的联合注册解剖(CT)和功能(SPECT)图。该仪器将允许9名NIH资助的研究人员完成并显著改进他们在药物开发、肿瘤进展分期和基因传递有效性监测方面的研究。重要的是，该仪器将使研究人员能够在不牺牲实验动物的情况下完成体内的纵向研究。因此，研究人员将研究更少的动物，但从每种动物那里获得更多的数据。X-SPECT(TM)成像系统能够对大量容易获得的单光子发射放射性同位素成像，如1-125(30keV)、TI-201(75keV)、1-123(159keV)、Ln-111(171,250keV)和1-131(364keV)。成像1-125的能力特别值得注意，因为1-125通常用于体外分子生物学研究，除标记各种药物外，还标记核酸、抗体和配体。新的药物也可以相对容易地加碘。有了X-SPECT(Tm)，这些研究现在可以在体内非侵入性地进行。此外，X-SPECT(Tm)成像系统可以同时进行双同位素成像，这样人们就可以使用两种不同的示踪剂同时观察药物输送和疗效。针孔准直器的使用使SPECT成像的空间分辨率低于1 mm。X射线CT系统的分辨率优于100？m。俄亥俄州立大学或俄亥俄州的其他研究中心没有类似的仪器。该仪器将被安置在怀斯曼大厅的放射性同位素认证的实验室动物设施中，由一名研究核医学技术专家监督，该专家将与内部咨询委员会密切合作。首席调查员和研究核医学技术员接受了使用A-SPECT(Tm)成像系统(X-SPECT1成像系统的微SPECT组件)的培训。用户将接受首席研究员、研究核医学技术员和实验室动物兽医的培训。该软件将在联网的计算机上的窗口环境中运行，使调查人员能够从遥远的地点访问数据。按小时收费将为年度服务合同提供资金。X-SPECT(Tm)系统将被整合为成像核心设施(共享资源)的一个组成部分。因此，仪器的空间和公用设施以及研究核医学技术人员的工资和福利将由俄亥俄州立大学综合癌症中心提供。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): An X-SPECT imaging system made by Gamma Medica, Inc. is requested in this Shared Instrument Grant application. X-SPECT(tm) is a dual-modality small animal imaging system that combines x-ray Computed Tomography (CT) and Single Photon Emission Computed Tomography (SPECT). Thus, X-SPECT(tm) is capable of generating co-registered anatomical (CT) and functional (SPECT) maps of intact animals under anesthesia. This instrument will allow 9 NIH- funded investigators to complete and significantly improve their studies on drug development, staging of tumor progression, and monitoring of gene delivery efficacy. Importantly, the instrument will enable the investigators to complete longitudinal studies in vivo without sacrifice of experimental animals. Consequently, investigators will study fewer animals but get much more data from each animal. The X-SPECT(tm) imaging system is capable of imaging large number of single photon emitting radioisotopes that are readily available, such as 1-125 (30 keV), TI-201 (75 keV), 1-123 (159 keV), ln-111 (171, 250 keV) and 1-131 (364 keV). The capability to image 1-125 is of particular noteworthy as 1-125 is commonly used in ex vivo molecular biology studies to label nucleic acids, antibodies, and ligands in addition to various Pharmaceuticals. New Pharmaceuticals can also be iodinated relatively easily. With X-SPECT(tm), these studies can now be performed non-invasively in vivo. In addition, the X-SPECT(tm) imaging system can perform dual isotope imaging simultaneously, such that one may look at drug delivery and efficacy simultaneously using two different tracers. The use of pinhole collimator allows the spatial resolution for SPECT imaging less than 1 mm. The x-ray CT system achieves high resolution better than 100 ¿m. There is no similar instrument at the Ohio State University or other research centers in the State of Ohio. The instrument will be housed in a radioisotope certified laboratory animal facility in Wiseman Hall under the supervision of a research nuclear medicine technologist, who will work closely with the internal advisory committee. The principal investigator and the research nuclear medicine technologist have received training in the use of the A-SPECT(tm) imaging system (microSPECT component of the X-SPECT1 imaging system). Users will be trained by the principal investigator, the research nuclear medicine technologist, and laboratory animal veterinarian. The software will operate in a Window environment on a networked computer that will allow the investigators to access the data from distant sites. An hourly fee will provide funds for the annual service contract. The X-SPECT(tm) system will be integrated as a component of an Imaging Core Facility (shared resource). Thus, space and utilities for the instrument plus the salary and benefits for the research nuclear medicine technologist will be provided by the Ohio State University Comprehensive Cancer Center.