Epigenetic toxicity of polycyclic aromatic hydrocarbons

多环芳烃的表观遗传毒性

• 批准号: 7147012
• 负责人: Brad L. Upham
• 金额: $ 35.86万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-16 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7147012
• 关键词: apoptosis; biological signal transduction; carbopolycyclic compound; cell cell interaction; cell differentiation; cell line; chromatography; environmental exposure; environmental toxicology; enzyme activity; epigenetics; gap junctions; gene expression; isomer; mitogen activated protein kinase; neoplastic growth; phospholipase inhibitor; proteomics; small interfering RNA; smoking; tobacco abuse

DESCRIPTION (provided by applicant): Considerable toxicological research on polycyclic aromatic hydrocarbons (PAHs), which are prevalent compounds in cigarette smoke that contribute to cancer, has focused on their genotoxic attributes. However, cancer is not solely the consequence of non-reversible mutagenic events but also include reversible, epigenetic events. Thus, there is a need to reassess the toxicity of PAHs at the epigenetic level. Gap junctional intercellular communication (GJIC) centrally modulates signal transduction pathways that epigenetically alters gene expression. There is considerable evidence linking abnormal regulation of GJIC with the nongenotoxic steps of tumor promotion. Mitogen activated protein kinases (MAPKs) also play a central role in cell signaling. We will use a series of environmental and tobacco smoke-relevant PAHs and determine structure-activity relationships with inter-and intracellular signaling mechanisms in pluripotent mammalian epithelial cell lines. We will specifically test the hypothesis SA#1 that phospholipases are the upstream regulators of GJIC and MAPK in response to PAHs by using specific phospholipase inhibitors and the emerging and powerful technique of silencing genes using small interfering RNA. We will use chromatography and state of the art proteomic techniques to test the hypothesis SA#2 that lipid-derived second messengers are released from the plasma membrane, and activate cell signal proteins. We will also test the hypothesis SA#3 that inhibition of GJIC and activation of MAPK by PAHs will induce mitogenesis, and block apoptosis and differentiation. We would like to note that the use of biologically active versus inactive PAH isomers for all of the aims allows us to systematically identify molecular events that are specific to the regulation of GJIC and MAPK and subtract out non-specific events. Overall, determining the effect of environmental and tobacco-relevant PAHs on key signaling events would provide invaluable mechanistically based information on the epigenetic toxicity of these compounds, thereby aiding in the development of preventative and therapeutic strategies for cancer.

描述（由申请人提供）：多环芳烃（PAHs）是香烟烟雾中普遍存在的导致癌症的化合物，其毒理学研究主要集中在其基因毒性特性上。然而，癌症不仅仅是不可逆转的突变事件的结果，还包括可逆的表观遗传事件。因此，有必要在表观遗传水平上重新评估多环芳烃的毒性。间隙连接细胞间通讯（GJIC）集中调节信号转导途径，表观遗传改变基因表达。有相当多的证据表明，GJIC的异常调节与肿瘤促进的非基因毒性步骤有关。丝裂原活化蛋白激酶（MAPKs）在细胞信号传导中也起着重要作用。我们将使用一系列环境和烟草烟雾相关的多环芳烃，并确定多能哺乳动物上皮细胞系中细胞间和细胞内信号机制的结构-活性关系。我们将通过使用特定的磷脂酶抑制剂和使用小干扰RNA沉默基因的新兴强大技术来具体验证假设SA#1，即磷脂酶是GJIC和MAPK响应多环芳烃的上游调节剂。我们将使用色谱和最先进的蛋白质组学技术来验证假设SA#2，即脂质衍生的第二信使从质膜释放，并激活细胞信号蛋白。我们还将验证假说SA#3，即多环芳烃抑制GJIC和激活MAPK会诱导有丝分裂，并阻断细胞凋亡和分化。我们想指出的是，在所有目标中使用生物活性和非活性多环芳烃异构体使我们能够系统地识别GJIC和MAPK调控的特异性分子事件，并减去非特异性事件。总的来说，确定环境和烟草相关的多环芳烃对关键信号事件的影响将为这些化合物的表观遗传毒性提供宝贵的基于机制的信息，从而有助于制定预防和治疗癌症的策略。