EPIGENIC TOXICITY OF POLYCYCLIC AROMATIC HYDROCARBONS

多环芳烃的表观毒性

• 批准号: 7359136
• 负责人: Brad L. Upham
• 金额: $ 4.07万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7359136
• 关键词: EPIGENIC; TOXICITY; POLYCYCLIC; AROMATIC; HYDROCARBONS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polycyclic aromatic hydrocarbons (PAHs) are abundant components of tobacco smoke that have been implicated in cancer formation. A large body of work indicates that these compounds induce genetic damage and this results cancer. However, cancer is not solely the consequence of non-reversible mutagenic events but also involves reversible, epigenetic events. Thus, there is a need to reassess the toxicity of PAHs at the epigenetic level. Gap junctional intercellular communication (GJIC) centrally modulates signal transduction pathways that epigenetically alters gene expression. There is considerable evidence linking abnormal regulation of GJIC with the non-genotoxic steps of tumor promotion. We have worked with the Upham laboratory to devise a proteomics approach to detect early changes in protein abundance and post-translational modification due to PAHs.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。多环芳烃 (PAH) 是烟草烟雾中的丰富成分，与癌症的形成有关。大量研究表明，这些化合物会引起遗传损伤，从而导致癌症。然而，癌症不仅是不可逆诱变事件的结果，而且还涉及可逆的表观遗传事件。因此，有必要在表观遗传水平上重新评估PAHs的毒性。间隙连接细胞间通讯（GJIC）集中调节信号转导途径，从而在表观遗传上改变基因表达。有大量证据表明 GJIC 的异常调节与肿瘤促进的非基因毒性步骤有关。我们与 Upham 实验室合作，设计了一种蛋白质组学方法来检测多环芳烃引起的蛋白质丰度和翻译后修饰的早期变化。