New cold-water enzymes from polar metagenomes

该项目将采用几种方法来寻找新的突破性酶用于洗涤剂和其他应用：(1)该项目将全面表征现有的候选酶及其结构/活性关系；(2)将收集新的样本以确定和产生新的候选酶文库；(3)将选择上述活动中出现的先导分子进行同源研究，涉及搜索公共数据库、大学现有的元基因组以识别相关分子，并产生用于表征和应用测试的各种变异体。最有希望的线索将被推进到小规模的消费者研究。

This project will pursue several approaches to identify new breakthrough enzymes for detergent and other applications: (1) the project will fully characterise an existing candidate enzyme and its structure/activity relationship, (2) new samples will be collected be mined to identify and produce new libraries of enzyme candidates and (3) lead molecules emerging from the above activities will be selected for homologue studies involving searches of public databases, the university's existing metagenomes to identify related molecules and produce a diverse panel of variants for characterisation and application testing. The most promising leads will be advanced to small-scale consumer research.