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Imaging islets in implantable microcapsules

可植入微胶囊中的胰岛成像

基本信息

批准号：
7290302
负责人：
BARJOR GIMI
金额：
$ 37.72万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-22 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7290302
关键词：
Affect Apoptotic Back Beta Cell Biocompatible Calcium Cell Death Cells Chicago Class Condition Confocal Microscopy Contrast Media Cytoplasm Data Depth Devices Diffusion Dose Dyes Elements Encapsulated Fluo 4 Fluorescence Free Radicals Frequencies Functional Imaging Fura-2 Future Generations Glucose Goals Heating Housing Human Image Individual Insulin Islet Cell Islets of Langerhans Islets of Langerhans Transplantation Magnetic Resonance Imaging Manganese Measures Membrane Potentials Methods Microcapsules drug delivery system Mitochondria Numbers Nutrient Optical Methods Optics Oxidation-Reduction Oxygen Pattern Photons Physiological Polymers Porosity Preparation Principal Investigator Process Publishing Radio Relaxation Reperfusion Injury Reporting Research Personnel Research Project Grants Sampling Solvents Standards of Weights and Measures Structure Surface Swelling Techniques Temperature Testing Time Transplantation Universities Waste Products Water Work base bench to bedside calcium indicator day density design fluorescence imaging ilium in vivo insulin granule insulin secretion islet melting mitochondrial dysfunction nanopore optical imaging programs release of sequestered calcium ion into cytoplasm response seal self assembly sensor size success time use uptake

项目摘要

DESCRIPTION (provided by applicant): The overall goal of our research project is to develop cubic microcapsules that can effectively encapsulate individual islets. Three orthogonal surfaces of the microcapsules will be nanoporous, to immunoprotect the islet cells from the host while allowing the free exchange of nutrients, waste products, glucose and insulin between the encapsulated islet cells and the host. The three nanoporous surfaces will also be transparent so as to optically probe the encapsulated islet's calcium fluxes and mitochondrial response to glucose, and test the hypothesis that encapsulation does not affect islet function. On the other three surfaces, we will mount radio frequency (RF) sensors for MRI. We will use MRI to image islet function using activation-based uptake of the contrast agent manganese. We will test the hypothesis that islet function and viability can be assessed through MRI, post encapsulation. The strength of our approach is that we can provide elements that are currently elusive in encapsulated islet cell therapy: 1) immunoprotection of islets from the host through extremely well-controlled porosity of the encapsulation device, not achieved with tortuous polymers, 2) a large surface-to-volume ratio that provides oxygen and nutrients to the individual islets, not achieved with present day MEMS based biocapsules, 3) direct imaging of the viability of encapsulated islets through optical methods, and 4) direct, noninvasive, periodic and functional imaging of the encapsulated islets. Our approach can be easily adapted to encapsulate any native or genetically modified beta cells, and our devices may be mounted with a plethora of sensors to measure oxygen, pH, temperature and other environmental conditions in vivo.

描述（由申请人提供）：