The molecular basis of daily and seasonal migration behaviour in the copepod Calanus finmarchicus in the face of climate change

面对气候变化，桡足类Calanus finmarchicus每日和季节性迁徙行为的分子基础

• 批准号: 2889087
• 金额: --
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2889087
• 关键词: molecular; basis; daily; seasonal; migration

Climate-change-induced increases in oceanic temperatures are causing poleward distribution range shifts in many temperate marine organisms. These shifts have far-reaching effects on marine food webs and oceanic carbon fluxes. Zooplankton are a central component of oceanic ecosystems, where they capture primary carbon fixed by phytoplankton and provide the major carbon source to higher trophic levels, including commercially important fish stocks. The cosmopolitan and lipid-rich copepod Calanus finmarchicus dominates zooplankton biomass in the North Atlantic and has emerged as a powerful model organism for understanding the biogeochemical processes in oceanic carbon cycling.Calanus displays two behaviours that are crucial for its role in the oceanic food web and carbon pump. First, diel vertical migration (DVM), which is a circadian behaviour whereby the animals dive to depth during the day and migrate to the surface to feed at night. This behaviour is a predator avoidance response and is driven primarily by light. Second, long resting stage (diapause), which is a crucial part of the life cycle of Calanus whereby juveniles in their last pre-adult copepodid stage in late spring migrate from their surface feeding grounds down to deeper epipelagic waters (~400-1000m) where they remain inactive for 7-8 months until January. The factors governing timing of diapause entry and exit remain unclear, but are suspected to include daylength, temperature and lipid reserves.Both DVM and diapause structure populations in space and time and both behaviours are sensitive to changes in environmental cues. Climate change is facilitating range expansions into higher latitudes where the cues (light, temperature) governing these behaviours are likely to differ from those at lower latitudes. Therefore, the capacity of individuals and populations to respond to these phenological shifts via phenotypic plasticity and/or adaptation will be a crucial factor in affecting realised range shifts, biomass flux and broader biogeochemical processes in the North Atlantic.A major hindrance in predicting population responses to phenological shifts is a lack of insight into the physiological and molecular processes affecting behavioural variability at the individual level. DVM timing, speed and depth vary measurably among individuals and show some correlation with among-individual variability in photo-responsiveness (unpublished ongoing work in KSL's group) and metabolic factors such as respiration rates and lipid reserves. Similarly, individuals vary in their timing of diapause entry, though diapause exit is often remarkably synchronised. Very little is known about the molecular genetic basis of this among-individual variation, of the physiological basis of behavioural synchronicity, and how adaptive genetic diversity among Calanus populations is structured in space and time.This project will examine the molecular genetic variation associated with variation in DVM and diapause behaviour and environmental factors likely to drive behavioural synchronicity. The principal aims are twofold: First, to examine the links between gene expression, behavioural phenotypic plasticity and environmental factors hypothesised to trigger and synchronise DVM and diapause. Second, to examine genetic diversity and genetic structure among populations from different latitudes from nearshore coastal to open ocean environments. We hypothesise that, first, environmental triggers of DVM/diapause cause changes in expression at key genes involved in biological rhythms consistent with phenotypic plasticity, and, second, that these genes show allele-frequency differences among populations from different latitudes, consistent with an adaptive evolutionary response to range shifts.

气候变化引起的海洋温度上升正在导致许多温带海洋生物的分布范围向极地转移。这些变化对海洋食物网和海洋碳通量产生深远影响。浮游动物是海洋生态系统的核心组成部分，它们捕获浮游植物固定的主要碳，并为较高营养级提供主要碳源，包括具有重要商业价值的鱼类种群。世界性的和富含脂质的桡足类Calanus finmarchicus占主导地位的浮游动物生物量在北大西洋，并已成为一个强大的模式生物，了解海洋碳循环的地球化学过程中的Calanus显示两个行为，是至关重要的，它在海洋食物网和碳泵的作用。首先，昼夜垂直迁移（DVM），这是一种昼夜节律行为，即动物在白天潜入深处，晚上迁移到水面觅食。这种行为是一种捕食者回避反应，主要由光驱动。第二，漫长的休眠期（滞育），这是哲水蚤生命周期的关键部分，在春季末，处于最后一个成年前桡足类阶段的幼鱼从其表面觅食地迁移到更深的上层沃茨（约400- 1000米），在那里它们保持7-8个月的不活动状态，直到1月。影响滞育进入和退出时间的因素尚不清楚，但怀疑包括日照长度，温度和脂质reserves. DVM和滞育结构种群在空间和时间，这两种行为是敏感的环境线索的变化。气候变化正在促进分布范围向高纬度扩展，在高纬度地区，控制这些行为的线索（光照、温度）可能与低纬度地区不同。因此，个体和种群通过表型可塑性和/或适应性对这些物候变化做出反应的能力将是影响北南极实现的范围变化、生物量通量和更广泛的生态地球化学过程的关键因素。预测种群对物候变化反应的一个主要障碍是缺乏对影响个体水平行为变异的生理和分子过程的洞察。DVM的时间、速度和深度在个体之间存在可测量的差异，并显示出与光反应性（KSL小组未发表的正在进行的工作）和代谢因素（如呼吸率和脂质储备）的个体差异的一些相关性。同样，个体进入滞育的时间也各不相同，尽管滞育的退出通常是显著同步的。目前对这种双个体变异的分子遗传基础、行为同步性的生理基础以及哲水蚤种群之间的适应性遗传多样性在空间和时间上是如何构建的知之甚少，本项目将研究与DVM和滞育行为变异相关的分子遗传变异以及可能驱动行为同步性的环境因素。主要目的是双重的：首先，研究基因表达，行为表型可塑性和环境因素之间的联系假设触发和同步DVM和滞育。第二，研究从近岸沿海到开阔海洋环境的不同纬度种群的遗传多样性和遗传结构。我们假设，第一，环境触发DVM/滞育引起的变化，参与生物节律的关键基因的表达与表型可塑性一致，第二，这些基因表现出等位基因频率的差异，从不同纬度的人群，符合适应性进化反应范围的变化。