Myosin Modifications in Aged Muscle

候选人：K02候选人LaDora V. Thompson是一位细胞生理学家，其研究是 专注于年龄引起的骨骼肌退化的分子机制。虽然 汤普森博士受过肌肉生理学的训练，在肌肉生物化学方面获得了经验， 分子生物物理学她的近期目标是集中精力在她资助的NIA R01赠款“结构 和钙调节蛋白在肌肉减少症中的作用”。她的长期目标是保持或超过 目前的生产力水平在以下几个方面：1）成功地实现持续的外部资助 研究重点是确定与年龄相关的肌肉的潜在分子机制， 功能障碍，2）通过利用最先进的生物物理学和 3）指导学生和博士后研究员进行老龄化研究。K02 独立科学家奖将为候选人提供必要的资金，以达到这些职业生涯 通过提供工资支持，以减少她的重大教学负担，让她花在目标 她的大部分时间都花在研究上。 环境：汤普森博士继续研究进展的环境是杰出的。她 研究合作者托马斯博士和费灵顿博士是国际知名的科学家， 肌肉生物物理学和蛋白质修饰（蛋白质组学）。汤普森、托马斯和费灵顿医生 参与资助的老龄化中心和肌肉和肌肉疾病中心， 明尼苏达大学。他们有成功的合作和出版物的历史。他们的 结合专业知识提供了一种新的方法来回答有关年龄的具体问题， 肌肉退化。 研究：本研究的总体目标是确定潜在的分子机制 随着年龄的增长，肌肉性能下降（目标1）。我们用电子顺磁共振 光谱学（目标2）和蛋白质组学（目标3）技术来研究年龄对结构的影响， 肌球蛋白是肌肉中主要的收缩蛋白。

Candidate: The K02 candidate, LaDora V. Thompson, is a cellular physiologist whose research is focused on molecular mechanisms underlying age-induced skeletal muscle deterioration. Although trained in muscle physiology, Dr. Thompson has acquired experience in muscle biochemistry and molecular biophysics. Her immediate goal is to focus intensively on her funded NIA R01 grant "Structural and Calcium Regulatory Proteins in Sarcopenia". Her long term goals are to maintain or exceed the current level of productivity in the following areas: 1) successfully attain continued extramurally-funded research focused on identifying underlying molecular mechanisms responsible for age-related muscle dysfunction, 2) continue to contribute to aging research by utilizing state of the art biophysical and )roteomic technology, 3) mentor students and post-doctoral fellows in aging research. The K02 Independent Scientist Award will provide the necessary funding for the candidate to reach these career goals by providing salary support to decrease her significant teaching load and allow her to spend the majority of her time on research. Environment: The environment for Dr. Thompson's continued research progress is outstanding. Her research collaborators, Drs. Thomas and Ferrington, are internationally known scientists in the fields of muscle biophysics and proteins modifications (proteomics). Drs. Thompson, Thomas and Ferrington are involved with the funded Center on Aging and the Center for Muscle and Muscle Disorders at the University of Minnesota. They have a history of successful collaborations and publications. Their combined expertise provides a novel approach to answering specific questions about age-related deterioration of muscle. Research: The overall aim of this research is to determine the molecular mechanisms underlying diminished performance of muscle with age (Aim 1). We are using electron paramagnetic resonance spectroscopy (Aim 2) and proteomic (Aim 3) techniques to study the effects of age on the structure, function, and post-translational modifications of myosin, the major contractile protein in muscle.